A functional approach toward xerogel immobilization for encapsulation biocompatibility of Rhizobium toward biosensor

Abstract

Sol-gel derived silica has tremendous applications as a biocompatible scaffold for the immobilization of cells. The use of xerogel as a matrix in the blueprint of biosensors is an appealing proposition due to several inimitable characteristics of xerogels, primarily because of their high porous nature, amendable pore size, and exceptionally large internal surface area. Morphological (X-Ray Diffraction and Thermogravimmetric Analysis) and optical (Fourier Transform Infrared and UV-Vis absorption) studies of the silica matrices with entrapped Rhizobial (Rz) structure of the biomaterial has been made. Temporal and concentration dependent studies were conducted for impregnated samples; it showed that the response time for the new biosensor for determining the concentration of Rz is less than 20 min. In this work, first time a novel avenue to create a generic approach for the fabrication of biosensor has been created.

Keywords

biosensor / Fourier Transform Infrared spectroscopy (FTIR) / Rhizobium / Thermo Gravimmetric Analysis (TGA) / sol-gel / xerogel / X-Ray Diffraction (XRD)

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Acknowledgments

One of the authors (S. Sharma) is grateful to Prof. Devendra Mohan, Guru Jambheshwar University of Science and Technology, Hisar, India and Dr. Reji Philip, Raman Research Institute, Bangalore for the useful discussions and providing the experimental facilities to carry out this work.

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg