Current technologies to identify protein kinase substrates in high throughput
Received date: 27 Dec 2012
Accepted date: 22 Jan 2013
Published date: 01 Apr 2013
Copyright
Since the discovery of protein phosphorylation as an important modulator of many cellular processes, the involvement of protein kinases in diseases, such as cancer, diabetes, cardiovascular diseases, and central nervous system pathologies, has been extensively documented. Our understanding of many disease pathologies at the molecular level, therefore, requires the comprehensive identification of substrates targeted by protein kinases. In this review, we focus on recent techniques for kinase substrate identification in high throughput, in particular on genetic and proteomic approaches. Each method with its inherent advantages and limitations is discussed.
Liang XUE , W. Andy TAO . Current technologies to identify protein kinase substrates in high throughput[J]. Frontiers in Biology, 2013 , 8(2) : 216 -227 . DOI: 10.1007/s11515-013-1257-z
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