Improvement of plant cryosection

Abstract

Cryosection in plants is usually challenging because of the larger amounts of water contained in plant cell than animal cell. The formation of ice crystals within the plant cells easily destroys subcellular detail during freeze processing. And cell walls make freezing plant tissues hardly sliced and preserve the cell structure in subtle ways. In this study, the technique was improved on infusion, embedding and flatting sections with the winter wheat Variety Aikang58 as raw material.

Key words： cryosection; wheat; histochemistry

Acknowledgments

The financial support provided by the National Basic Research of China (2012CB114300), the National Natural Science Foundation of China (Grant No. 31101232), 12th Five-Year Plan of National Science and Technology Support, China (2011BAD07B02) was greatly appreciated.

References

1 Asan E (2003). Innovative techniques and applications in histochemistry and cell biology. Histochem Cell Biol, 120(6): 523-548

PMID

2 Baltaci S, Adsan O, Ugurlu O, Aslan G, Can C, Gunaydin G, Buyukalpelli R, Elhan A H, Beduk Y (2011). Reliability of frozen section examination of obturator lymph nodes and impact on lymph node dissection borders during radical cystectomy: results of a prospective multicentre study by the Turkish Society of Urooncology. BJU Int, 107(4): 547-553

PMID

3 Bernier R, Iglesias R, Simard R (1972). Detection of DNA by tritiated actinomycin D on ultrathin frozen sections. J Cell Biol, 53(3): 798-808

PMID

4 Gal A A (2005). The centennial anniversary of the frozen section technique at the Mayo Clinic. Arch Pathol Lab Med, 129(12): 1532-1535

PMID

5 Jacqmard A, Detry N, Dewitte W, Van Onckelen H, Bernier G (2002). In situ localisation of cytokinins in the shoot apical meristem of Sinapis alba at floral transition. Planta, 214(6): 970-973

PMID

6 Jensen A J, Naik A M, Pommier R F, Vetto J T, Troxell M L (2010). Factors influencing accuracy of axillary sentinel lymph node frozen section for breast cancer. Am J Surg, 199(5): 629-635

PMID

7 Kang E J, Cho J Y, Lee T H, Jin S Y, Cho W Y, Bok J H, Kim H G, Kim J O, Lee J S, Lee I H (2011). Frozen section biopsy to evaluation of obscure lateral resection margins during gastric endoscopic submucosal dissection for early gastric cancer. J Gastric Cancer, 11(3): 155-161

PMID

8 Liu J F, Cheng Y Q, Yan X F, Zhou G, Wang S F (2006). Improvement of plant cryo-sectioning technique. J Nanjing Forestry Uni, 30: 128-130

9 Morin X K, Soll J (1997). Immunogold labelling of cryosectioned pea chloroplasts and initial localization of the proteins associated with the protein import machinery. Planta, 201(2): 119-127

10 Ripper D, Schwarz H, Stierhof Y D (2008). Cryo-section immunolabelling of difficult to preserve specimens: advantages of cryofixation, freeze-substitution and rehydration. Biol Cell, 100(2): 109-123

PMID

11 Stierhof Y D, El Kasmi F (2010). Strategies to improve the antigenicity, ultrastructure preservation and visibility of trafficking compartments in Arabidopsis tissue. Eur J Cell Biol, 89(2-3): 285-297

PMID

12 Tokuyasu K T (1973). A technique for ultracryotomy of cell suspensions and tissues. J Cell Biol, 57(2): 551-565

PMID

13 Turhan N, Ozgüler Z, Cağlı K, Cağlı K, Gölbaşı Z (2011). Primary cardiac undifferentiated sarcoma: role of intraoperative imprint cytology and frozen section of two cases. Cardiovasc Pathol, 20(4): 232-237

PMID

14 Vitha S, Osteryoung K W (2011). Immunofluorescence microscopy for localization of Arabidopsis chloroplast proteins. Methods Mol Biol, 774: 33-58

PMID

15 Ye H, Kong X, He T W, Jolis T, Choi K, Lepor H, Melamed J (2011). Intraoperative frozen section analysis of urethral margin biopsies during radical prostatectomy. Urology, 78(2): 399-404

PMID