RESEARCH ARTICLE

Improvement of plant cryosection

  • Zhaopei ZHANG 1 ,
  • Liyuan NIU 1 ,
  • Xiling CHEN 2 ,
  • Xu XU 1 ,
  • Zhengang RU , 3
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  • 1. Lab Center, Henan Institute of Science and Technology, Xinxiang 453003, China
  • 2. College of Resources and Environmental Sciences, Henan Institute of Science and Technology, Xinxiang 453003, China
  • 3. Wheat Improvement Center, Henan Institute of Science and Technology, Xinxiang 453003, China

Received date: 17 Apr 2012

Accepted date: 15 May 2012

Published date: 01 Aug 2012

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

Cryosection in plants is usually challenging because of the larger amounts of water contained in plant cell than animal cell. The formation of ice crystals within the plant cells easily destroys subcellular detail during freeze processing. And cell walls make freezing plant tissues hardly sliced and preserve the cell structure in subtle ways. In this study, the technique was improved on infusion, embedding and flatting sections with the winter wheat Variety Aikang58 as raw material.

Cite this article

Zhaopei ZHANG , Liyuan NIU , Xiling CHEN , Xu XU , Zhengang RU . Improvement of plant cryosection[J]. Frontiers in Biology, 2012 , 7(4) : 374 -377 . DOI: 10.1007/s11515-012-2127-9

Acknowledgments

The financial support provided by the National Basic Research of China (2012CB114300), the National Natural Science Foundation of China (Grant No. 31101232), 12th Five-Year Plan of National Science and Technology Support, China (2011BAD07B02) was greatly appreciated.
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