Improvement of plant cryosection

Abstract

Cryosection in plants is usually challenging because of the larger amounts of water contained in plant cell than animal cell. The formation of ice crystals within the plant cells easily destroys subcellular detail during freeze processing. And cell walls make freezing plant tissues hardly sliced and preserve the cell structure in subtle ways. In this study, the technique was improved on infusion, embedding and flatting sections with the winter wheat Variety Aikang58 as raw material.

Keywords

cryosection / wheat / histochemistry

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Acknowledgments

The financial support provided by the National Basic Research of China (2012CB114300), the National Natural Science Foundation of China (Grant No. 31101232), 12th Five-Year Plan of National Science and Technology Support, China (2011BAD07B02) was greatly appreciated.

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg