Media optimization for extracellular amylase production by Pseudomonas balearica vitps19 using response surface methodology
Received date: 12 Dec 2017
Accepted date: 02 Feb 2018
Published date: 28 May 2018
Copyright
BACKGROUND: In this study, we optimized the process for enhancing amylase production from Pseudomonas balearica VITPS19 isolated from agricultural lands in Kolathur, India.
METHODS: Process optimization for enhancing amylase production from the isolate was carried out by Response Surface Methodology (RSM) with optimized chemical and physical sources using Design expert v.7.0. A central composite design was used to evaluate the interaction between parameters. Interaction between four factors – maltose (C-source), malt extract (N-source), pH, and CaCl2 was studied.
RESULTS: The factors pH and CaCl2 concentration were found to affect amylase production. Validation of the experiment showed a nearly twofold increase in alpha amylase production.
CONCLUSION: Amylase production was thus optimized and increased yield was achieved.
Moni Philip Jacob Kizhakedathil , Subathra Devi Chandrasekaran . Media optimization for extracellular amylase production by Pseudomonas balearica vitps19 using response surface methodology[J]. Frontiers in Biology, 2018 , 13(2) : 123 -129 . DOI: 10.1007/s11515-018-1485-3
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