RESEARCH ARTICLE

Media optimization for extracellular amylase production by Pseudomonas balearica vitps19 using response surface methodology

  • Moni Philip Jacob Kizhakedathil ,
  • Subathra Devi Chandrasekaran
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  • Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore – 632014, Tamil Nadu, India

Received date: 12 Dec 2017

Accepted date: 02 Feb 2018

Published date: 28 May 2018

Copyright

2018 Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature

Abstract

BACKGROUND: In this study, we optimized the process for enhancing amylase production from Pseudomonas balearica VITPS19 isolated from agricultural lands in Kolathur, India.

METHODS: Process optimization for enhancing amylase production from the isolate was carried out by Response Surface Methodology (RSM) with optimized chemical and physical sources using Design expert v.7.0. A central composite design was used to evaluate the interaction between parameters. Interaction between four factors – maltose (C-source), malt extract (N-source), pH, and CaCl2 was studied.

RESULTS: The factors pH and CaCl2 concentration were found to affect amylase production. Validation of the experiment showed a nearly twofold increase in alpha amylase production.

CONCLUSION: Amylase production was thus optimized and increased yield was achieved.

Cite this article

Moni Philip Jacob Kizhakedathil , Subathra Devi Chandrasekaran . Media optimization for extracellular amylase production by Pseudomonas balearica vitps19 using response surface methodology[J]. Frontiers in Biology, 2018 , 13(2) : 123 -129 . DOI: 10.1007/s11515-018-1485-3

Acknowledgements

We are greatly indebted to the Vellore Institute of Technology for their constant encouragement, help, and support in extending the necessary facilities.
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