Media optimization for extracellular amylase production by Pseudomonas balearica vitps19 using response surface methodology

Abstract

BACKGROUND: In this study, we optimized the process for enhancing amylase production from Pseudomonas balearica VITPS19 isolated from agricultural lands in Kolathur, India.

METHODS: Process optimization for enhancing amylase production from the isolate was carried out by Response Surface Methodology (RSM) with optimized chemical and physical sources using Design expert v.7.0. A central composite design was used to evaluate the interaction between parameters. Interaction between four factors – maltose (C-source), malt extract (N-source), pH, and CaCl₂ was studied.

RESULTS: The factors pH and CaCl₂ concentration were found to affect amylase production. Validation of the experiment showed a nearly twofold increase in alpha amylase production.

CONCLUSION: Amylase production was thus optimized and increased yield was achieved.

Keywords

Pseudomonas balearica VITPS19 / alpha amylase / optimization / response surface methodology / central composite design / pH

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Acknowledgements

We are greatly indebted to the Vellore Institute of Technology for their constant encouragement, help, and support in extending the necessary facilities.

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