RESEARCH ARTICLE

Evaluation of microRNA-146a expression in acute lymphoblastic leukemia

  • Farzaneh Tavakoli 1 ,
  • Kaveh Jaseb 1 ,
  • Mohammad Ali Jalali Far 1 ,
  • Masoud Soleimani 2 ,
  • Elahe Khodadi 1 ,
  • Najmaldin Saki , 1
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  • 1. Health Research Institute, Research Center of Thalassemia & Hemoglobinopathy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  • 2. Department of hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Received date: 16 Dec 2015

Accepted date: 26 Jan 2016

Published date: 22 Mar 2016

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

MicroRNAs (miRNAs) play an essential role in the development and progression of acute lymphoblastic leukemia (ALL), and could serve as disease biomarkers and therapeutic targets. The function of miR-146a in lymphoid differentiation has been here with discussed. However, the role of this miRNA in the outcome of ALL is not well understood. Peripheral blood of 48 patients with ALL and 20 age- and sex-matched healthy control subjects was used to accurately evaluate the expression of miR-146a by stem-loop Real time PCR. No statistically significant difference was found between patients and controls in total miR-146a expression. The expression of miR-146a was high (18.75%), low (27.08%) and not different (54.17%) in ALL patients. Our analysis indicated no association between the expression of miR-146a and any prognostic factors such as WBC/PLT counts, Hb, fusion genes (P190 and some translocations) with ALL type. This study revealed that miR-146a cannot be an independent factor for predicting the outcome of ALL patients. We suggest a multi-parameter analysis including miRNAs, transcription factors and critical genes to achieve a precise clinical panel for prognostic values.

Cite this article

Farzaneh Tavakoli , Kaveh Jaseb , Mohammad Ali Jalali Far , Masoud Soleimani , Elahe Khodadi , Najmaldin Saki . Evaluation of microRNA-146a expression in acute lymphoblastic leukemia[J]. Frontiers in Biology, 2016 , 11(1) : 53 -58 . DOI: 10.1007/s11515-016-1387-1

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