Comparative analysis of panicle proteomes of two upland rice varieties upon hyper-osmotic stress

Abstract

Drought is a major environmental factor that limits the yield of rice dramatically. Upland rice is now regarded as a promising rice cultivar in water saving agriculture. Two varieties of upland rice Zhonghan 3 and IR29 were used to compare the physiological and proteomic responses to hyper-osmotic stress induced by 15% polyethyleneglycol (PEG) at the reproductive stage. Osmotic stress affected the growth development and caused the loss of production especially the grain yield. IR29 was more tolerant to PEG than Zhonghan 3 as shown by less yield loss under osmotic stress conditions. Comparative proteomic analysis of the panicle suggested that the up-regulation of glycolysis related proteins and defense proteins may contribute to the better osmotic tolerance in IR29.

Key words： upland rice; panicle proteome; grain yield; glycolysis; cytosolic ascorbate peroxidase

Acknowledgements

This work was supported by the National High Technology Research and Development Program of China (No. 2007AA100603), the International Science & Technology Cooperation Program of China (No. 2010DFA91930) and a project from the Ministry of Agriculture of China for transgenic research (No. 2008ZX08009).

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