Comparative analysis of panicle proteomes of two upland rice varieties upon hyper-osmotic stress

Abstract

Drought is a major environmental factor that limits the yield of rice dramatically. Upland rice is now regarded as a promising rice cultivar in water saving agriculture. Two varieties of upland rice Zhonghan 3 and IR29 were used to compare the physiological and proteomic responses to hyper-osmotic stress induced by 15% polyethyleneglycol (PEG) at the reproductive stage. Osmotic stress affected the growth development and caused the loss of production especially the grain yield. IR29 was more tolerant to PEG than Zhonghan 3 as shown by less yield loss under osmotic stress conditions. Comparative proteomic analysis of the panicle suggested that the up-regulation of glycolysis related proteins and defense proteins may contribute to the better osmotic tolerance in IR29.

Keywords

upland rice / panicle proteome / grain yield / glycolysis / cytosolic ascorbate peroxidase

References

[1] Abbasi F M, Komatsu S (2004). A proteomic approach to analyze salt-responsive proteins in rice leaf sheath. Proteomics, 4(7): 2072-2081

[2] Agrawal G K, Rakwal R (2006). Rice proteomics: a cornerstone for cereal food crop proteomes. Mass Spectrom Rev, 25(1): 1-53

[3] Bernier J, Atlin G N, Serraj R, Kumar A, Spaner D (2008). Breeding upland rice for drought resistance. J Sci Food Agric, 88(6): 927-939

[4] Bernier J, Kumar A, Ramaiah V, Spaner D, Atlin G (2007). A large-effect QTL for grain yield under reproductive-stage drought stress in upland rice. Crop Sci, 47(2): 507-516

[5] Del Buono D, Prinsi B, Espen L, Scarponi L (2009). Triosephosphate isomerases in Italian ryegrass (Lolium multiflorum ): characterization and susceptibility to herbicides. J Agric Food Chem, 57(17): 7924-7930

[6] Douce R, Bourguignon J, Neuburger M, Rébeillé F (2001). The glycine decarboxylase system: a fascinating complex. Trends Plant Sci, 6(4): 167-176

[7] Jiang C J, Shoji K, Matsuki R, Baba A, Inagaki N, Ban H, Iwasaki T, Imamoto N, Yoneda Y, Deng X W, Yamamoto N (2001). Molecular cloning of a novel importin alpha homologue from rice, by which constitutive photomorphogenic 1 (COP1) nuclear localization signal (NLS)-protein is preferentially nuclear imported. J Biol Chem, 276(12): 9322-9329

[8] Johansson H, Sterky F, Amini B, Lundeberg J, Kleczkowski L A (2002). Molecular cloning and characterization of a cDNA encoding poplar UDP-glucose dehydrogenase, a key gene of hemicellulose/pectin formation. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1576: 53-58

[9] Jubault M, Hamon C, Gravot A, Lariagon C, Delourme R, Bouchereau A, Manzanares-Dauleux M J (2008). Differential regulation of root arginine catabolism and polyamine metabolism in clubroot-susceptible and partially resistant Arabidopsis genotypes. Plant Physiol, 146(4): 2008-2019

[10] Király Z (1998). Plant infection-biotic stress. Ann N Y Acad Sci, 851(STRESS OF LIFE: FROM MOLECULES TO MAN): 233-240

[11] Liu G L, Mei H W, Yu X Q, Zou G H, Liu H Y, Li M S, Chen L, Wu J H, Luo L J (2007). Panicle water potential, a physiological trait to identify drought tolerance in rice. J Integr Plant Biol, 49(10): 1464-1469

[12] Liu H Y, Mei H W, Yu X Q, Zou G H, Liu G L, Luo L J (2006). Towards improving the drought tolerance of rice in China. Plant Genetic Resources, 4(1): 47-53

[13] Lu Z, Liu D, Liu S (2007). Two rice cytosolic ascorbate peroxidases differentially improve salt tolerance in transgenic Arabidopsis. Plant Cell Rep, 26(10): 1909-1917

[14] Lu Z, Neumann P M (1999). Water stress inhibits hydraulic conductance and leaf growth in rice seedlings but not the transport of water via mercury-sensitive water channels in the root. Plant Physiol, 120(1): 143-152

[15] Peng Z Y, Wang M C, Li F, Lv H J, Li C L, Xia G M (2009). A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat. Mol Cell Proteomics, 8(12): 2676-2686

[16] Pillai M A, Lihuang Z, Akiyama T (2002). Molecular cloning, characterization, expression and chromosomal location of OsGAPDH, a submergence responsive gene in rice (Oryza sativa L.). Theor Appl Genet, 105(1): 34-42

[17] Rabello A R, Guimarães C M, Rangel P H, da Silva F R, Seixas D, de Souza E, Brasileiro A C, Spehar C R, Ferreira M E, Mehta A (2008). Identification of drought-responsive genes in roots of upland rice (Oryza sativa L). BMC Genomics, 9(1): 485

[18] Riccardi F, Gazeau P, Zivy Mde Vienne D, Zivy M (1998). Protein changes in response to progressive water deficit in maize. Quantitative variation and polypeptide identification. Plant Physiol, 117(4): 1253-1263

[19] Salekdeh G H, Siopongco J, Wade L J, Ghareyazie B, Bennett J (2002). A proteomic approach to analyzing drought- and salt-responsiveness in rice. Field Crops Res, 76(2-3): 199-219

[20] Shigeoka S, Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K (2002). Regulation and function of ascorbate peroxidase isoenzymes. J Exp Bot, 53(372): 1305-1319

[21] Singla-Pareek S L, Yadav S K, Pareek A, Reddy M K, Sopory S K (2006). Transgenic tobacco overexpressing glyoxalase pathway enzymes grow and set viable seeds in zinc-spiked soils. Plant Physiol, 140(2): 613-623

[22] Smirnoff N (1998). Plant resistance to environmental stress. Curr Opin Biotechnol, 9(2): 214-219

[23] Sun Z X, Cheng S H, Si H MZongxiu S, Shihua C, Huamin S (1993). Determination of critical temperatures and panicle development stage for fertility change of thermo-sensitive genic male sterile rice line '5460S'. Euphytica, 67(1-2): 27-33

[24] Suzuki K, Watanabe K, Masumura T, Kitamura S (2004). Characterization of soluble and putative membrane-bound UDP-glucuronic acid decarboxylase (OsUXS) isoforms in rice. Arch Biochem Biophys, 431(2): 169-177

[25] Suzuki M, Hashioka A, Munyra T, Ashihara H (2005). Salt stress and glycolytic regulation in suspension-cultured cells of the mangrove tree, Bruguiera sexangula. Physiol Plant,123(3): 246-253

[26] Taylor N L, Day D A, Millar A H (2002). Environmental stress causes oxidative damage to plant mitochondria leading to inhibition of glycine decarboxylase. J Biol Chem, 277(45): 42663-42668

[27] Thornalley P J (1990). The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life. Biochem J, 269(1): 1-11

[28] Vauclare P, Diallo N, Bourguignon J, Macherel D, Douce R (1996). Regulation of the expression of the glycine decarboxylase complex during pea leaf development. Plant Physiol, 112(4): 1523-1530

[29] Venuprasad R, Lafitte H R, Atlin G N (2007). Response to direct selection for grain yield under drought stress in rice. Crop Sci, 47(1): 285-293

[30] Xiao X, Yang Y, Yang Y, Lin J, Tang D, Liu X (2009). Comparative analysis of young panicle proteome in thermo-sensitive genic male-sterile rice Zhu-1S under sterile and fertile conditions. Biotechnol Lett, 31(1): 157-161

[31] Xie J H, Zapata-Arias F J, Shen M, Afza R (2000). Salinity tolerant performance and genetic diversity of four rice varieties. Euphytica, 116(2): 105-110

[32] Yadav S K, Singla-Pareek S L, Ray M, Reddy M K, Sopory S K (2005a). Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione. Biochem Biophys Res Commun, 337(1): 61-67

[33] Yadav S K, Singla-Pareek S L, Reddy M K, Sopory S K (2005b). Transgenic tobacco plants overexpressing glyoxalase enzymes resist an increase in methylglyoxal and maintain higher reduced glutathione levels under salinity stress. FEBS Lett, 579(27): 6265-6271

[34] Yan S P, Tang Z C, Su W A, Sun W N (2005). Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics, 5(1): 235-244

[35] Yan S P, Zhang Q Y, Tang Z C, Su W A, Sun W N (2006). Comparative proteomic analysis provides new insights into chilling stress responses in rice. Mol Cell Proteomics, 5(3): 484-496

[36] Yang S L, Lan S S, Gong M (2009). Hydrogen peroxide-induced proline and metabolic pathway of its accumulation in maize seedlings. J Plant Physiol, 166(15): 1694-1699

[37] Yue B, Xue W Y, Xiong L Z, Yu X Q, Luo L J, Cui K H, Jin D M, Xing Y Z, Zhang Q F (2006). Genetic basis of drought resistance at reproductive stage in rice: separation of drought tolerance from drought avoidance. Genetics, 172(2): 1213-1228

[38] Zang X, Komatsu S (2007). A proteomics approach for identifying osmotic-stress-related proteins in rice. Phytochemistry, 68(4): 426-437

[39] Zhang Q S, Shirley N, Lahnstein J, Fincher G B (2005). Characterization and expression patterns of UDP-D-glucuronate decarboxylase genes in barley. Plant Physiol, 138(1): 131-141

Acknowledgements

This work was supported by the National High Technology Research and Development Program of China (No. 2007AA100603), the International Science & Technology Cooperation Program of China (No. 2010DFA91930) and a project from the Ministry of Agriculture of China for transgenic research (No. 2008ZX08009).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg