RESEARCH ARTICLE

Prostaglandin E2 promotes hematopoietic development from human embryonic stem cells

  • Chao YANG 1,2 ,
  • Jia-Fei XI 1 ,
  • Xiao-Yan XIE 1 ,
  • Wen YUE 1 ,
  • Ruo-Yong WANG 1 ,
  • Qiong WU 1 ,
  • Li-Juan HE 1 ,
  • Xue NAN 1 ,
  • Yan-Hua LI 1 ,
  • Xue-Tao PEI , 1
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  • 1. Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China
  • 2. 522 Hospital of Chinese PLA, Luoyang 471000, China

Received date: 29 Aug 2010

Accepted date: 16 Sep 2010

Published date: 01 Oct 2010

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

Recent studies have suggested that prostaglandin (PG) E2 (PGE2) and the prostaglandin pathway are essential for hematopoietic stem cell growth and development. However, similar studies on hematopoietic commitment from human embryonic stem cells (hESCs) are still limited. Here we report that the addition of PGE2 promotes hematopoietic differentiation of hESCs. The induced cells from hESCs/OP9 co-culture and in the presence of PGE2 were characterized by reverse transcription-PCR (RT-PCR), flow cytometry, colony-forming assays and Wright-Giemsa staining. Our results demonstrated that PGE2 exposure could alter the gene expression pattern and morphology of co-cultured hESCs and resulted in a robust hematopoietic differentiation with higher frequencies of CD34+ and CD45+ cells. Furthermore, the Smad signaling pathway may be involved in PGE2 and OP9 induced hematopoietic differentiation of hESCs. This research may improve our knowledge of stem cell regulation and hopefully lead to better stem cell-based therapeutic options.

Cite this article

Chao YANG , Jia-Fei XI , Xiao-Yan XIE , Wen YUE , Ruo-Yong WANG , Qiong WU , Li-Juan HE , Xue NAN , Yan-Hua LI , Xue-Tao PEI . Prostaglandin E2 promotes hematopoietic development from human embryonic stem cells[J]. Frontiers in Biology, 2010 , 5(5) : 445 -454 . DOI: 10.1007/s11515-010-0810-2

Acknowledgements

This work was supported by the National High Technology Research and Development Program of China (No: 2006AA02A107), the State Key Program for Basic Research of China (No: 2005CB522702), and the Project of Beijing Municipal Science & Technology Commission (No: D07050701350702).
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