REVIEW

Bioreactor technology for clonal propagation of plants and metabolite production

  • Nazmul H. A. Mamun 1 ,
  • Ulrika Egertsdotter 1,2 ,
  • Cyrus K. Aidun , 1,3
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  • 1. G.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
  • 2. Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden
  • 3. Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA

Received date: 17 Dec 2014

Accepted date: 02 Mar 2015

Published date: 06 May 2015

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

Plant cell culture in bioreactors is an enabling tool for large scale production of clonal elite plants in agriculture, horticulture, forestry, pharmaceutical sectors, and for biofuel production. Advantages of bioreactors for plant cell culture have resulted in various types of bioreactors differing in design, operating technologies, instrumentations, and construction of culture vessels. In this review, different types of bioreactors for clonal propagation of plants and secondary metabolites production are discussed. Mechanical and biochemical parameters associated with bioreactor design, such as aeration, flow rate, mixing, dissolved oxygen, composition of built-up gas in the headspace, and pH of the medium, are pivotal for cell morphology, growth, and development of cells within tissues, embryos, and organs. The differences in such parameters for different bioreactor designs are described here, and correlated to the plant materials that have been successfully cultured in different types of bioreactors.

Cite this article

Nazmul H. A. Mamun , Ulrika Egertsdotter , Cyrus K. Aidun . Bioreactor technology for clonal propagation of plants and metabolite production[J]. Frontiers in Biology, 2015 , 10(2) : 177 -193 . DOI: 10.1007/s11515-015-1355-1

Compliance with ethics guidelines

Nazmul H. A. Mamun, Ulrika Egertsdotter, and Cyrus K. Aidun declare that they have no conflict of interest.
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