REVIEW

Analyzing stem cell dynamics: use of cutting edge genetic approaches in model organisms

  • Gary R. HIME , 1 ,
  • Nicole SIDDALL 1 ,
  • Katja HORVAY 2 ,
  • Helen E. ABUD 2
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  • 1. Department of Anatomy and Neuroscience, University of Melbourne, VIC 3010, Australia
  • 2. Department of Anatomy and Developmental Biology, Monash University, Clayton VIC 3800, Australia

Received date: 31 Oct 2014

Accepted date: 05 Jan 2015

Published date: 14 Feb 2015

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

Regeneration of many cell types found in adult organs relies upon the presence of relatively small pools of undifferentiated stem cells. Initial studies that attempted to isolate stem cells and propagate them in vitro have been complemented by analysis of stem cells in their endogenous tissues where they are subject to a variety of regulatory cues. This has been facilitated by the advent of new methods for lineage tracing and genetic manipulation of stem cells and their associated niche cells. The picture that is emerging is that different stem cell populations utilize diverse processes to ensure maintenance of the stem cell pool accompanied by production of cells committed to regenerate differentiated cells.

Cite this article

Gary R. HIME , Nicole SIDDALL , Katja HORVAY , Helen E. ABUD . Analyzing stem cell dynamics: use of cutting edge genetic approaches in model organisms[J]. Frontiers in Biology, 2015 , 10(1) : 1 -10 . DOI: 10.1007/s11515-015-1347-1

Acknowledgments

The authors would like to acknowledge the assistance of the Bloomington Drosophila Stock Center and the Australian Drosophila Biomedical Research Support Facility. GRH and HEA received funding from NHMRC(the National Medical Research Council of Australia) Grants APP1048110 and APP1011187, and GRH is also supported by Discovery Project Grant 120100224 from the Australian Research Council.
Compliance with ethics guidelines
Gary Hime, Nicole Siddall, Katja Horvay and Helen Abud declare that they have no conflict of interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.
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