Research progress on anthocyanin-based intelligent pH indicator films

Lin Zhang , Chunxue Zhang , Xinmei Yun , Ahmed Adel Ashour , Xinyu Wang , Hui Tan , Xinyao Jiao

Food Innovation and Advances ›› 2026, Vol. 5 ›› Issue (3) : 492 -502.

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Food Innovation and Advances ›› 2026, Vol. 5 ›› Issue (3) :492 -502. DOI: 10.48130/fia-0026-0039
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Research progress on anthocyanin-based intelligent pH indicator films
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Abstract

With the continuous consumer demands for the improvement of food safety, quality, and freshness preservation, food packaging technology is rapidly evolving. Although traditional packaging materials have barrier and protective functions, they lack the capability for real-time monitoring of food quality and transmitting information. Intelligent packaging, as a novel technological system, not only ensures food safety but also enables intuitive monitoring of food quality. Natural pigments represented by anthocyanins have become a cutting-edge direction in research into intelligent packaging films because of their remarkable pH sensitivity and multiple biological activities. This paper systematically reviews the chemical structure and physicochemical properties of anthocyanins, and the selection of substrate materials and film-forming mechanisms for intelligent packaging films. The functional characteristics and application examples of anthocyanin-based intelligent pH indicator films in meat products, aquatic products, fruits, vegetables, and dairy products are discussed. In light of research from the past five years, the advantages of being eco-friendly and enabling visual monitoring of food's freshness, and the limitations of poor stability and susceptibility to environmental interference of anthocyanin-based intelligent packaging films are analyzed in this review. Future development trends are presented, aiming to provide a reference for subsequent research and industrial applications.

Keywords

Anthocyanins / pH indicator films / Intelligent packaging / Food preservation

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Lin Zhang, Chunxue Zhang, Xinmei Yun, Ahmed Adel Ashour, Xinyu Wang, Hui Tan, Xinyao Jiao. Research progress on anthocyanin-based intelligent pH indicator films. Food Innovation and Advances, 2026, 5 (3) : 492-502 DOI:10.48130/fia-0026-0039

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References

[1]

Roy S, Rhim JW. 2021. Anthocyanin food colorant and its application in pH—responsive color change indicator films. Critical Reviews in Food Science and Nutrition 61(14): 2297-2325

[2]

Shi C, Zhang J, Jia Z, Yang X, Zhou Z. 2021. Intelligent pH indicator films containing anthocyanins extracted from blueberry peel for monitoring tilapia fillet freshness. Journal of the Science of Food and Agriculture 101(5): 1800-1811

[3]

Roy S, Deshmukh RK, Tripathi S, Gaikwad KK, Das SS, et al. 2023. Recent advances in the carotenoids added to food packaging films: a review. Foods 12(21): 4011

[4]

Zhang K, Huang TS, Yan H, Hu X, Ren T. 2020. Novel pH—sensitive films based on starch/polyvinyl alcohol and food anthocyanins as a visual indicator of shrimp deterioration. International Journal of Biological Macromolecules 145: 768-776

[5]

Gao R, Hu H, Shi T, Bao Y, Sun Q, et al. 2022. Incorporation of gelatin and Fe2+ increases the pH—sensitivity of zein—anthocyanin complex films used for milk spoilage detection . Current Research in Food Science 5: 677-686

[6]

Jiang G, Hou X, Zeng X, Zhang C, Wu H, et al. 2020. Preparation and characterization of indicator films from carboxymethyl—cellulose/starch and purple sweet potato (Ipomoea batatas (L.) lam) anthocyanins for monitoring fish freshness . International Journal of Biological Macromolecules 143: 359-372

[7]

Gonzali S, Perata P. 2020. Anthocyanins from purple tomatoes as novel antioxidants to promote human health. Antioxidants 9(10): 1017

[8]

Prietto L, Pinto VZ, El Halal SLM, de Morais MG, Costa JAV, et al. 2018. Ultrafine fibers of zein and anthocyanins as natural pH indicator. Journal of the Science of Food and Agriculture 98(7): 2735-2741

[9]

Luo Q, Zhang Y, Zhou Y, Liu SG, Gao W, et al. 2021. Portable functional hydrogels based on silver metallization for visual monitoring of fish freshness. Food Control 123: 107824

[10]

Agarwal S, Greiner A, Wendorff JH. 2013. Functional materials by electrospinning of polymers. Progress in Polymer Science 38(6): 963-991

[11]

Ge Y, Li Y, Bai Y, Yuan C, Wu C, et al. 2020. Intelligent gelatin/oxidized chitin nanocrystals nanocomposite films containing black rice bran anthocyanins for fish freshness monitorings. International Journal of Biological Macromolecules 155: 1296-1306

[12]

Ulrich S, Moura SO, Diaz Y, Clerc M, Guex AG, et al. 2020. Electrospun colourimetric sensors for detecting volatile amines. Sensors and Actuators B: Chemical 322: 128570

[13]

Li Y, Wu K, Wang B, Li X. 2021. Colorimetric indicator based on purple tomato anthocyanins and chitosan for application in intelligent packaging. International Journal of Biological Macromolecules 174: 370-376

[14]

Kumar TSM, Kumar KS, Rajini N, Siengchin S, Ayrilmis N, et al. 2019. A comprehensive review of electrospun nanofibers: food and packaging perspective. Composites Part B: Engineering 175: 107074

[15]

Khoo HE, Azlan A, Tang ST, Lim SM. 2017. Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food & Nutrition Research 61: 1361779

[16]

Wang L, Lan W, Chen D. 2024. Blueberry (Vaccinium spp.) anthocyanins and their functions, stability, bioavailability, and applications . Foods 13(17): 2851

[17]

Fang H, Yin X, He J, Xin S, Zhang H, et al. 2022. Cooking methods affected the phytochemicals and antioxidant activities of potato from different varieties. Food Chemistry: X 14: 100339

[18]

Boudergua S, Belaidi S, AlMogren MM, Bounif A, Bakhouch M, et al. 2023. QSAR modeling using the Gaussian process applied for a series of flavonoids as potential antioxidants. Journal of King Saud University — Science 35(8): 102898

[19]

Bizjak J, Jakopic J, Slatnar A, Stampar F, Stich K, et al. 2012. Late prohexadione—calcium application on maturing apple cv. 'Braeburn' fruit reduces anthocyanins and alters the phenolic content. European Journal of Horticultural Science 77(4): 154-162

[20]

Sadilova E, Carle R, Stintzing FC. 2007. Thermal degradation of anthocyanins and its impact on color and in vitro antioxidant capacity . Molecular Nutrition & Food Research 51(12): 1461-1471

[21]

Lee GY, Lim KJ, Lee YH, Shin HS. 2024. Development of a freshness indicator for assessing the quality of packaged pork products during refrigerated storage. Foods 13(13): 2097

[22]

Pham TN, Toan TQ, Lam TD, Hieu VQ, Vo DVN, et al. 2019. Anthocyanins extraction from Purple Sweet Potato (Ipomoea Batatas (L.) Lam): the effect of pH values on natural color . IOP Conference Series: Materials Science and Engineering 542: 012031

[23]

Luchese CL, Abdalla VF, Spada JC, Tessaro IC. 2018. Evaluation of blueberry residue incorporated cassava starch film as pH indicator in different simulants and foodstuffs. Food Hydrocolloids 82: 209-218

[24]

Mei LX, Nafchi AM, Ghasemipour F, Easa AM, Jafarzadeh S, et al. 2020. Characterization of pH sensitive sago starch films enriched with anthocyanin—rich torch ginger extract. International Journal of Biological Macromolecules 164: 4603-4612

[25]

Samadi S, Ghasemnezhad A, Imani J. 2017. Extending shelf life of strawberry using some pre—storage treatments.Acta Horticulturae 1156: 643-652

[26]

Yang W, Guo Y, Liu M, Chen X, Xiao X, et al. 2022. Structure and function of blueberry anthocyanins: a review of recent advances. Journal of Functional Foods 88: 104864

[27]

Tan S, Zhou C, Rao P, Tan H, Wang J. 2025. Antioxidant pH—sensitive films incorporating CMC/SA/starch, anthocyanins, and tea polyphenols for monitoring freshness of pork. Meat Science 225: 109808

[28]

Chen K, Li J, Li L, Wang Y, Qin Y, et al. 2023. A pH indicator film based on sodium alginate/gelatin and plum peel extract for monitoring the freshness of chicken. Food Bioscience 53: 102584

[29]

Li Y, Li Z, Wang Y, Sun L, Pei HC. 2023. Anthocyanins/chitosan films doped by nano zinc oxide for active and intelligent packaging: comparison of anthocyanins source from purple tomato or black wolfberry. Frontiers of Chemical Science and Engineering 17(6): 704-715

[30]

Huang HL, Tsai IL, Lin C, Hang YH, Ho YC, et al. 2023. A Intelligent films of marine polysaccharides and purple cauliflower extract for food packaging and spoilage monitoring. Carbohydrate Polymers 299: 120133

[31]

Alizadeh—Sani M, Tavassoli M, Mohammadian E, Ehsani A, Khaniki GJ, et al. 2021. pH—responsive color indicator films based on methylcellulose/chitosan nanofiber and barberry anthocyanins for real—time monitoring of meat freshness. International Journal of Biological Macromolecules 166: 741-750

[32]

Yan J, Cui R, Qin Y, Li L, Yuan M. 2021. A pH indicator film based on chitosan and butterfly pudding extract for monitoring fish freshness. International Journal of Biological Macromolecules 177: 328-336

[33]

Fernández—Marín R, Fernandes SCM, Sánchez MÁA, Labidi J. 2022. Halochromic and antioxidant capacity of smart films of chitosan/chitin nanocrystals with curcuma oil and anthocyanins. Food Hydrocolloids 123: 107119

[34]

Su Y, Guerra NB, Herculano RD, Mussagy CU. 2025. Sketching deep eutectic solvents for the development of halochromic smart packaging using blueberry residue anthocyanins and alginate—based films for monitoring food freshness. International Journal of Biological Macromolecules 321: 146525

[35]

Yang H, Li L, Li C, Xu Z, Tao Y, et al. 2024. Multifunctional and antimicrobial carboxymethyl cellulose—based active hydrogel film for fruits packaging and preservation. Food Bioscience 59: 104005

[36]

Zhao R, Guan W, Zheng P, Tian F, Zhang Z, et al. 2022. Development of edible composite film based on chitosan nanoparticles and their application in packaging of fresh red sea bream fillets. Food Control 132: 108545

[37]

Huang X, Zhao W, Li Z, Zhang N, Wang S, et al. 2023. Preparation of a dual—functional active film based on bilayer hydrogel and red cabbage anthocyanin for maintaining and monitoring pork freshness. Foods 12(24): 4520

[38]

Elhadef K, Chaari M, Akermi S, Ennouri K, Ben Hlima H, et al. 2024. Gelatin—sodium alginate packaging film with date pits extract: an eco—friendly packaging for extending raw minced beef shelf life. Meat Science 207: 109371

[39]

Wen P, Wu J, Wu J, Wang H, Wu H. 2024. A colorimetric nanofiber film based on ethyl cellulose/gelatin/purple sweet potato anthocyanins for monitoring pork freshness. Foods 13(5): 717

[40]

Pasanako P, Somsaard W, Yotnarong O, Laksee S, Tangthong T, et al. 2025. pH—sensitive film based on gelatin and anthocyanin extracts incorporated with gamma irradiation for real—time monitoring of beef freshness. Materials Today Communications 46: 112314

[41]

Meng F, Zhang C, Li J, Wang L. 2021. Self—assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gumfilm . International Journal of Biological Macromolecules 167: 1281-1289

[42]

Li Y, Tang X, Zhu L. 2022. Bilayer pH—sensitive colorimetric indicator films based on zein/gellan gum containing black rice (Oryza sativa L.) extracts for monitoring of largemouth bass (Micropterus salmoides) fillets freshness . International Journal of Biological Macromolecules 223: 1268-1277

[43]

Yong H, Wang X, Bai R, Miao Z, Zhang X, et al. 2019. Development of antioxidant and intelligent pH—sensing packaging films by incorporating purple—fleshed sweet potato extract into chitosan matrix. Food Hydrocolloids 90: 216-224

[44]

Capello C, Leandro GC, Gagliardi TR, Valencia GA. 2021. Intelligent films from chitosan and biohybrids based on anthocyanins and laponite®: physicochemical properties and food packaging applications. Journal of Polymers and the Environment 29(12): 3988-3999

[45]

Yong H, Liu J. 2020. Recent advances in the preparation, physical and functional properties, and applications of anthocyanins—based active and intelligent packaging films. Food Packaging and Shelf Life 26: 100550

[46]

Ran R, Wang L, Su Y, He S, He B, et al. 2021. Preparation of pH—indicator films based on soy protein isolate/bromothymol blue and methyl red for monitoring fresh—cut apple freshness. Journal of Food Science 86(10): 4594-4610

[47]

Khan S, Bao ZM, Abdullah, Wang J, Zhou X, et al. 2026. Smart funoran film incorporated with blueberry anthocyanins as a dual pH/NH3 sensor for real—time monitoring of shrimp freshness . Food Hydrocolloids 171: 111788

[48]

Luchesi BR, Moreira FKV, Marconcini JM. 2025. Colorimetric sensors based on hibiscus anthocyanins produced by continuous solution casting. Journal of Food Engineering 392: 112476

[49]

Gasti T, Dixit S, D'Souza OJ, Hiremani VD, Vootla SK, et al. 2021. Smart biodegradable films based on chitosan/methylcellulose containing Phyllanthus reticulatus anthocyanin for monitoring the freshness of fish fillet . International Journal of Biological Macromolecules 187: 451-461

[50]

Lawton JW. 2002. Zein: a history of processing and use. Cereal Chemistry 79(1): 1-18

[51]

Xu M, Fang D, Shi C, Xia S, Wang J, et al. 2025. Anthocyanin—loaded polylactic acid/quaternized chitosan electrospun nanofiber as an intelligent and active packaging film in blueberry preservation. Food Hydrocolloids 158: 110586

[52]

Yang Y, Shi Y, Cao X, Liu Q, Wang H, et al. 2021. Preparation and functional properties of poly(vinyl alcohol)/ethyl cellulose/tea polyphenol electrospun nanofibrous films for active packaging material. Food Control 130: 108331

[53]

Aghaei Z, Ghorani B, Emadzadeh B, Kadkhodaee R, Tucker N. 2020. Protein—based halochromic electrospun nanosensor for monitoring trout fish freshness. Food Control 111: 107065

[54]

Guo B, Sun Y, Guan Q, Luo Z, Zhou L, et al. 2024. Fabrication and characterization of sodium alginate/blueberry anthocyanins/hinokitiol loaded ZIF—8 nanoparticles composite films with antibacterial activity for monitoring pork freshness. Food Chemistry 440: 138200

[55]

Abdullah ZW, Dong Y, Davies IJ, Barbhuiya S. 2017. PVA, PVA blends, and their nanocomposites for biodegradable packaging application. Polymer—Plastics Technology and Engineering 56(12): 1307-1344

[56]

Chen Q, Zhang P, You N, Xu Y, Zhang Y, et al. 2023. Preparation and characterization of corn starch—based antimicrobial indicator films containing purple corncob anthocyanin and tangerine peel essential oil for monitoring pork freshness. International Journal of Biological Macromolecules 251: 126320

[57]

Zhang J, Zhang J, Huang X, Arslan M, Shi J, et al. 2023. Fabrication and characterization of polyvinyl alcohol/sodium alginate/zein/chitosan bilayer film for dynamic visualization of pork quality. International Journal of Biological Macromolecules 243: 125065

[58]

Zeng F, Ye Y, Liu J, Fei P. 2023. Intelligent pH indicator composite film based on pectin/chitosan incorporated with black rice anthocyanins for meat freshness monitoring. Food Chemistry: X 17: 100531

[59]

Li H, Zhang X, Li C. 2025. Preparation and characterization of intelligent films based on gellan gum/highly stable dual—modified anthocyanins for monitoring chicken freshness. International Journal of Biological Macromolecules 319: 145371

[60]

Guo H, Luo H, Huang C, Zheng J, Liu F, et al. 2025. High loading of anthocyanin on chitosan films by acrolein for sensitive monitoring of meat freshness. Food Chemistry 477: 143468

[61]

Vedove TMARD, Maniglia BC, Tadini CC. 2021. Production of sustainable smart packaging based on cassava starch and anthocyanin by an extrusion process. Journal of Food Engineering 289: 110274

[62]

Mu L, Bi J, Zhao H, Li J, Hou HM, et al. 2025. Intelligent pH—responsive films based on natural blueberry anthocyanins: a non—destructive monitoring system for the freshness of aquatic products with prospective smartphone compatibility. Food Chemistry: X 28: 102587

[63]

Yu Y, Xu J, Xu J, Li Y, Zhang X, et al. 2025. Preparation and characterization of chitosan/corn starch based films loaded withVaccinium vitis—Idaea anthocyanin nano complexes and the application in shrimp preservation . International Journal of Biological Macromolecules 303: 140734

[64]

Qiu L, Zhang M, Huang M, Chitrakar B, Chang L. 2023. Fabrication of sodium alginate/apricot peel pectin films incorporated with rose anthocyanin—rich extract for monitoring grass carp (Ctenopharyngodon idellus) freshness . Food Packaging and Shelf Life 39: 101150

[65]

Shayan M, Gwon J, Koo MS, Lee D, Adhikari A, et al. 2022. pH—responsive cellulose nanomaterial films containing anthocyanins for intelligent and active food packaging. Cellulose 29(18): 9731-9751

[66]

Liu J, Huang J, Ying Y, Hu L, Hu Y. 2021. pH—sensitive and antibacterial films developed by incorporating anthocyanins extracted from purple potato or roselle into chitosan/polyvinyl alcohol/nano—ZnO matrix: comparative study. International Journal of Biological Macromolecules 178: 104-112

[67]

Hao R, Pang S, Mraz J, Geng Y, Liu Y, et al. 2024. Anthocyanin modified by chondroitin sulphate and tannic acid improved the quality—indicating properties of gelatin—based intelligent film. Food Chemistry: X 24: 101779

[68]

Qin Y, Yun D, Xu F, Chen D, Kan J, et al. 2021. Smart packaging films based on starch/polyvinyl alcohol and Lycium ruthenicum anthocyanins—loaded nano—complexes: functionality, stability and application . Food Hydrocolloids 119: 106850

[69]

Hoffmann TG, Angioletti BL, Bertoli SL, de Souza CK. 2022. Intelligent pH—sensing film based on jaboticaba peels extract incorporated on a biopolymeric matrix. Journal of Food Science and Technology 59(3): 1001-1010

[70]

Muñoz—Coyotecatl S, Domínguez—Uscanga A, Ortiz—Castro R, Rosas—Saito GH, de la Vega GR, et al. 2025. Complex coacervation of anthocyanin—rich pigments from red cabbage (Brassica oleracea) with inulin, gum arabic and pea protein . Frontiers in Nutrition 12: 1523365

[71]

Li Z, Gao Z, Su D, Su Z, Fu Y, et al. 2025. Facile and green synthesis of nanocarriers from chondroitin sulfate—based and whey protein isolate with improved stability and biocompatibility for anthocyanins protection. Food Chemistry 476: 143449

[72]

Miao Y, Chen Z, Zhang J, Li N, Wei Z, et al. 2023. Exopolysaccharide riclin and anthocyanin—based composite colorimetric indicator film for food freshness monitoring. Carbohydrate Polymers 314: 120882

[73]

Zhu Y, Su Y, Hu L, Li Z, Xie T, et al. 2025. pH—responsive zein/chitosan composite film containing cinnamon essential oil—loaded Pickering emulsion and black wolfberry anthocyanin: Physicochemical properties, and application in packing salmon. Food Chemistry 479: 143815

[74]

Yan J, Cui R, Tang Z, Wang Y, Wang H, et al. 2021. Development of pH—sensitive films based on gelatin/chitosan/nanocellulose and anthocyanins from hawthorn (Crataegus scabrifolia) fruit . Journal of Food Measurement and Characterization 15(5): 3901-3911

[75]

Loukri A, Kyriakoudi A, Oliinychenko Y, Stratakos AC, Lazaridou A, et al. 2024. Preparation and characterization of chitosan—citric acid edible films loaded with Cornelian cherry pomace extract as active packaging materials. Food Hydrocolloids 150: 109687

[76]

Haghighi H, De Leo R, Bedin E, Pfeifer F, Siesler HW, et al. 2019. Comparative analysis of blend and bilayer films based on chitosan and gelatin enriched with LAE (lauroyl arginate ethyl) with antimicrobial activity for food packaging applications. Food Packaging and Shelf Life 19: 31-39

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