Synergistic ball milling-sonication extraction: impact on structural properties and lipid-binding activity of Agaricus bisporus chitosan

Abstract

In this study, dried Agaricus bisporus powder was pretreated using ball milling and sonication processing, followed by alkaline treatment to obtain Agaricus bisporus chitosan products. The yield of chitosan products obtained by this method can reach up to 5.67% ± 0.21%, with a purity of 85.54% ± 1.36% and a deacetylation degree of 82.86% ± 7.16%. However, the yield of chitosan products from traditional methods was only 2.13% ± 0.17%, with a purity of 44.51% ± 2.07% and a deacetylation degree (DD) of just 33.14% ± 5.41%. Furthermore, the results of the in vitro evaluation of lipid-binding activity show that chitosan obtained utilizing the ball-milling-sonication method exhibits superior lipid-binding activity. Specifically, its pancreatic lipase inhibitory rate was 85.15% ± 2.76%, and its adsorption capacities for oil, cholesterol micelles, sodium glycocholate hydrate, and sodium taurocholate were 5.52 ± 0.75 g/g, 13.07 ± 0.33 mg/g, 8.91 ± 0.56 mg/g and 4.87 ± 0.39 mg/g, respectively. Consequently, the chitosan preparation process utilized in this study possesses the potential to enhance the lipid-binding efficacy of Agaricus bisporus.

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