Frontiers of Forestry in China >

2007 , Vol. 2 >Issue 1: 78 - 81

DOI: https://doi.org/10.1007/s11461-007-0012-5

RAPD analysis of genetic relationships among Sphaeropsis sapinea isolates

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  • 1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.Jiangsu Forestry Administration, Nanjing 210036, China;

Published date: 05 Mar 2007

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Abstract

Genetic relationships were studied among 23 isolates of Sphaeropsis sapinea collected from China, the United States, England, South Africa and Chile by using a random amplification of a polymorphic DNA (RAPD) analytical method. One hundred and 35 DNA fragments were amplified with 12 random primers by a polymerase chain reaction PCR technique and 96.3% were polymorphic. The genetic dendrogram based on RAPD analysis showed that the S. sapinea isolates could be divided into three types. Isolate CWS41 from Chile was separated genetically as the first type that was different from other isolates and isolates F2 and J2 from China comprised the second group. The third RAPD group accommodated other isolates including the B morphotype isolate CWS43 from the United States.

Cite this article

WU Xiaoqin, WANG Yu, XIONG Dabin . RAPD analysis of genetic relationships among Sphaeropsis sapinea isolates[J]. Frontiers of Forestry in China, 2007 , 2(1) : 78 -81 . DOI: 10.1007/s11461-007-0012-5

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