Investigation of anti-salt stress on tetraploid Robinia pseudoacacia

Abstract

Tetraploid Robinia pseudoacacia was used as a main test material and diploid R. pseudoacacia was used as the control. The indices of shape, physiology and biochemistry, photosynthesis and anatomic structure of the young plants were investigated under salt stress (NaCl and Na₂SO₄). The treatment time was 30 d with an interval time of 7 d. Before and after treatment, the indices were measured. Results show that: 1) the growth of diploid R. pseudoacacia inhibited an evident symptom of salt damage and the leaf moisture content was lower under salt stress than that of control. But the tetraploid R. pseudoacacia was contrary. 2) The relative electric conductivity and proline (Pro) of tetraploid R. pseudoacacia increased slightly and had no significant difference compared with its control, which was contrary to diploid R. pseudoacacia. At the same time, three protective enzymes including perocidase (POD), superoxide (SOD) and catalase (CAT) kept higher activities at a post stage of salt stress to tetraploid R. pseudoacacia, which enhanced its anti-salt characteristics. Diploid R. pseudoacacia was sensitive to salt and had contrary information. 3) Salt stress had little influence to photosynthesis of tetraploid R. pseudoacacia. The net photosynthetic rate (P_n) and intercellular CO₂ concentration (C_i) had no significant changes, but those of diploid R. pseudoacacia decreased singificantly. 4) After salt stress, the anatomic structure of tetraploid R. pseudoacacia had a positive reaction, including the palisade parenchyma of diachyma, was prolonged and arranged more tightly. The spongy parenchyma was shrunk and was arranged tightly, which was contrary with diploid R. pseudoacacia. These data demonstrate that tetraploid R. pseudoacacia had superior anti-salt performance.

Keywords

tetraploid Robinia pseudoacacia / diploid Robinia pseudoacacia / salt stress / salt resistance

References

[1] Alscher R G, Alscher J L, Donahue K L(1997). Reactive oxygen species and antioxidants: relationships in green cells. Physiol Plant, 100: 224-233

[2] Asada K (1997). The role of ascorbate peroxidase and monodehydroascorbate reductase in H₂O₂ scavenging in plants. In: Scandalios J G, eds. Oxidative Stress and The Molecular Biology of Antioxidant Defence. New York: Cold Spring Harbor Laboratory Press, 715-735

[3] Shanghai Plant Physiology Research Institute of Chinese Academy of Sciences and Shanghai Society for Plant Physiology(2004). Guidebook mordern plant physiology experiments. Beijing: Science Press, 23-25 (in Chinese)

[4] Dai S X, Chen S L, Eberhard F, Andrea O, Christine K, Andrea P, Aloys H (2006). Ion compartmentation in leaf cells of Populus euphratica and P. tomentosa under salt stress. J Beijing For Univ, 28(2):1-5 (in Chinese)

[5] Dionisio S, Tobita M L (1998). Antioxidant responses of rice seedlings to salinity stress. Plant Sci, 135: 1-9

[6] Dong M F, Yuang W J, Shang F D (2005). Relations between anatomic structures of vegetative organs of the Llungiella halophila and its brackish environment. Acta Bot Boreal-Occident Sin, 25(6): 1077-1082 (in Chinese)

[7] Duan D Y, Liu X J, Fen F L , Li C Z (2003). Effect of salinities on seed germination of halophyte suaeda salsa. Chin Agr Sci B, 19(3): 168-172 (in Chinese)

[8] Fadzilla N M, Finch R P, Burdon R H (1997). Salinity, oxidative stress and antioxidant responses in shoot cultures of rice. J Exp Bot, 48(307): 325–33

[9] Farquhar G D, Sharkey T D (1982). Stomatal conductance and photosynthesis. Annu Rev Plant Phys, 33: 317–345

[10] Garratt L C, Janagoudar B S , Lowe K C (2002). Salinity tolerance and antioxidant status in cotton cultures. Free Radic Biol Med, 33: 502-511

[11] Guo W M, Fu Y P , Sun Z X, Zheng Z Y (2003). The correlation analysis between the morphological indices and salt tolerance in different rice germplasm under the salt stress. J Plant Genet Res, 4(3): 245-251 (in Chinese)

[12] Hong X Z, Eduardo B (2001). Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol, 19: 765-768

[13] Hou J T, Karil Y L, Dili X T, Maihe M T (2005). Effects of NaCl stress on microstructure of almond rootstocks. Chin Agr Sci B, 21(7): 258-261

[14] Huang L H, Liang Z W (2007). Effect of different sodium salt stress on the seed germination of tall wheatgrass (Agropyron elongatum). J Arid Land Res Environ, 21(6):174-176 (in Chinese)

[15] Li C Z, Liu X J, Huang W, Qiao H L (2005). Effect of salt stress on seed germination and its recovery of Atriplex centralasiatica. J Agr Univ Hebei, 28(6): 1-4 (in Chinese)

[16] Li G Q, An S Q, Zhagn J L, Li J, Wang Y J (2003). The anatomical characteristics of Populus deltoides (LUX) under stress of various soil salinities. Sci Silv Sin, 39(4): 89-98 (in Chinese)

[17] Li L, Wei X H , Wang H F (2007). Effect of salt stress physical activities of tobacco seedling. Seed, 26(5): 79-83 (in Chinese)

[18] Li Y, Jiang J Z (2006). Research progress of feed tetraploid black locust. Prat Sci, 23 (1): 41–46 (in Chinese)

[19] Lin S Z, Lin Y X, Zhang Z Y, Yong J,Zhang Q (2002). Response of antioxidant defense system in Populus tomentosa cuttings subjected to salt stress. For Stud China, 4(2): 16–20 (in Chinese)

[20] Liu J X, Wu X M, He T, Zhang W J (2004). Study of ultrastructure of Phragmites communismesophyll cell under salt stress. Acta Bot Boreal-Occident Sin, 24(6): 1035-1040 (in Chinese)

[21] Lu Y (2004). Tetraploid Robinia pseudoacacia. Agr Knowl, 12: 32 (in Chinese)

[22] McCord J M (2000). The evolution of free radicals and oxidative stress. Am J Med, 108: 652-659

[23] Meneguzzo S, Navari-Izzo F, Izzo R (1999). Antioxidative responses of shoots and roots of wheat to increasing NaCl concentrations. Plant Phys, 155: 274-280

[24] Mittler R (2002). Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci, 7: 405-410

[25] Munns R (2002).Comparative physiology of salt and water stress. Plant Cell Environ, 25: 239-250

[26] Pichu R (2006). World salinization with emphasis on Australia. J Exp Bot, 57(5): 1017-1023

[27] Qiu L Z, Hang Y J, Huang J Q, Xia G H, Gong J (2006). Comparative study on vegetal and physiological characteristics of different salt-tolerant plants under salt stress. J Zhejiang Univ, 32(4): 420-427 (in Chinese)

[28] Ren M T (2003). The biology character and economic value on tetraploid Robinia pseudoacacia. For Shanxin, 5: 29 (in Chinese)

[29] Sairam R K, Rao K V, Srivastava G C (2002). Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci, 163: 1037-1046

[30] Shi B S, Liu DY, Meng X S, Nie Q J, Wu J M, Du S H, Zhang Y G (2007). Physiological characteristic changes during the process of seed germination of Artemisia halodendron under NaCl, Na₂SO₄ stress. J Northwest For Univ, 22(5): 45-48 (in Chinese)

[31] Shi Y L (2004). Study on Desertificaion and Water-Land Resource in Westnorth Area. Beijing: Science Press, 3 (in Chinese)

[32] Tian Y T, Li Y (2003). The introduce of new Robinia pseudoacacia species. Sci Water Soil Conserv, 1(7): 54-59 (in Chinese)

[33] Wang R G, Chen S L, Liu L Y, Hao Z Y, Wen H J, Li H, Yang S, Duan S (2005). Genotypic difference in antioxidative ability and salt tolerance of three poplars under sact stress. J Beijing For Univ, 27(3): 46-52 (in Chinese)

[34] Wang W, Huang S L, Ding G J, Huang Y J, Huang J Q, Zheng B S (2007). Morphological and physiological changes accompanying the induction of salt tolerance in Neolitsea sericea seedlings. J Zhejiang For Coll, 24(2): 168-172 (in Chinese)

[35] Wei C X, Wang J J, Wang J B, Zhou W D, Sun G R, Liang J S (2006). Effects of Na₂CO₃ stress on the ultrastructure of mesophyll cells in Puccinellia tenuiflora. Acta Ecol Sin, 26(1): 108-114 (in Chinese)

[36] Xia F C, Jiang G Q, Lu J M (2002). Development of the study on Halophyte’s structure of resisting saline-alkali. J Tonghiia Teachers’Coll, 23(2): 67-69 (in Chinese)

[37] Xu X Q, Yang M S (2006). The present situation of Robinia pseudoacacia resource. J Hebei For Sci Tech, 9: 54-57 (in Chinese)

[38] Zhang Z L, Zhai W J (2003).Guide of Plant Physiology Experiments. Beijing: Higher Education Press, 20 (in Chinese)

[39] Zheng G C (1979). Micro-Technology of Biology. Beijing: People’s Education Press, 1 (in Chinese)

[40] Zhu X G, Zhang Q D (1999). Advances in the research on the effects of NaCl on photosynthesis. Chin Bull Bot, 16 (4): 332-338 (in Chinese)

[41] Zuo Z R, Gao J P, Mu D, Li C (2006). A comparative electron microscopic of ultra structure in different lily cultivars under salt stress. Acta Hort Sin, 33 (2): 429-432 (in Chinese)

Acknowledgements

This work was supported by the Research Award Fund for Outstanding Young Teachers in the Northeast Forestry University (No. 602026) and the Heilongjiang Postdoctoral Sustrntation Fund, China (No. LBH-Z06146).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg