Preparation and in vitro cytotoxicity study of poly(aspartic acid) stabilized magnetic nanoparticles

Abstract

Biocompatible magnetic nanoparticles were prepared by co-precipitation method in the presence of poly(aspartic acid) (PAsp) as stabilizer, which was one of the most extensively studied and used poly(amino acids). As a biocompatible dispersant, PAsp was successfully attached to the Fe₃O₄ nanoparticles, which was approved by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and thermogravimetric analysis (TGA). From X-ray diffraction (XRD) and vibrating sample magnetometry (VSM) measurement results, it was found that PAsp stabilized iron oxide nanoparticles possess excellent Fe₃O₄ crystal structure and superparamagnetic property. Compared with trisodium citrate stabilized magnetic nanoparticles, PAsp stabilized magnetic nanoparticles were biocompatible and with lower cytotoxicity, which makes it more applicable in medicine, biology and biomaterial science.

Keywords

poly(aspartic acid) (PAsp) / magnetic nanoparticles / biocompatible, in vitro, cytotoxicity

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant Nos. 20974023, 21034003 and 51073040) and Shanghai Committee of Science and Technology, China (Grant No. 10XD1400500).

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg