Effect of fatty acid methyl esters from plastrum testudinis on proliferation of rat bone mesenchymal stem cells

ZHANG Yuehua1, ZENG Heping2, CHEN Dongfeng3

Front. Chem. China ›› 2008, Vol. 3 ›› Issue (3) : 262-266.

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Front. Chem. China ›› 2008, Vol. 3 ›› Issue (3) : 262-266. DOI: 10.1007/s11458-008-0063-7

Effect of fatty acid methyl esters from plastrum testudinis on proliferation of rat bone mesenchymal stem cells

  • ZHANG Yuehua1, ZENG Heping2, CHEN Dongfeng3
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Abstract

The ointment of plastrum testudinis was extracted using petroleum ether, ether and dichloromethane sequentially and the extracts were methyl-esterified. The effects on the proliferation of bone marrow mesenchymal stem cells (bMMSCs) were examined by MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay and flow cytometry analysis. The volatile components of the samples were studied by gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC). The results show that the methyl-esterified parts can promote the proliferation of stem cells and they all contain palmitic acid methyl ester. Palmitic acid methyl ester can promote proliferation when the concentration was 0.15 ?g/?L. It may be concluded that the palmitic acid methyl ester is important for the methyl-esterified parts that have effects on proliferation.

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ZHANG Yuehua, ZENG Heping, CHEN Dongfeng. Effect of fatty acid methyl esters from plastrum testudinis on proliferation of rat bone mesenchymal stem cells. Front. Chem. China, 2008, 3(3): 262‒266 https://doi.org/10.1007/s11458-008-0063-7

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