Separation, purification and structure elucidation of a polysaccharide from root of

Abstract

A water-soluble polysaccharide (CPS-0) was obtained from the root of Cudrania tricuspidata (Carr.) Bur., by hot water extraction (70°C), deproteination using enzymolysis and Sevag method, precipitation with ethanol, and fractionation through DEAE-Sephadex A-50 chromatography. The purity of CPS-0 was determined by HPLC and the structure was elucidated by monosaccharide composition analysis, methylation analysis, GC, GC-MS, NMR spectral (¹H-NMR, ¹³C-NMR, HMQC), UV, IR, and elemental analysis. The CPS-0 was found to contain glucose residues only. The average repeating unit is a decasaccharide having a backbone consisting of 1,4-linked ?-D-glucopyranosyl residues to which the side chain consisting of terminal and 1,4-linked ?-D-glucopyranosyl residues was attached at position 6 of the branching residues.

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