%A FANG Jinian, DING Kan, DONG Qun, SHI Lei, CHEN Kaoshan
%T Separation, purification and structure elucidation
of a polysaccharide from root of
%0 Journal Article
%D 2008
%J Front. Chem. China
%J Frontiers of Chemistry in China
%@ 1673-3495
%R 10.1007/s11458-008-0040-1
%P 209-212
%V 3
%N 2
%U {https://journal.hep.com.cn/fcc/EN/10.1007/s11458-008-0040-1
%8 2008-06-05
%X A water-soluble polysaccharide (CPS-0) was obtained from the root of Cudrania tricuspidata (Carr.) Bur., by hot water extraction (70°C), deproteination using enzymolysis and Sevag method, precipitation with ethanol, and fractionation through DEAE-Sephadex A-50 chromatography. The purity of CPS-0 was determined by HPLC and the structure was elucidated by monosaccharide composition analysis, methylation analysis, GC, GC-MS, NMR spectral (1H-NMR, 13C-NMR, HMQC), UV, IR, and elemental analysis. The CPS-0 was found to contain glucose residues only. The average repeating unit is a decasaccharide having a backbone consisting of 1,4-linked ?-D-glucopyranosyl residues to which the side chain consisting of terminal and 1,4-linked ?-D-glucopyranosyl residues was attached at position 6 of the branching residues.