A study of using luminophore-doped silica nanoparticles as fluorescent probe in protein microarray assay

doi:10.1007/s11458-007-0040-6

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Front. Chem. China ›› 2007, Vol. 2 ›› Issue (2) : 199-203. DOI: 10.1007/s11458-007-0040-6

A study of using luminophore-doped silica nanoparticles as fluorescent probe in protein microarray assay

LIU Haibo¹, CHEN Chengxiang¹, TAN Fang¹, ZHUANG Zhixia², WANG Xiaoru², YANG Huanghao³, YAN Qingpi⁴

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Abstract

A convenient method for the synthesis of tris-(2,2´-bipyridyl) dichlororuthenium(II) hexahydrate-doped amino-modified double-layer silica nanoparticles is presented in this paper. The synthesized nanoparticles are uniform and photostable, and can be well dispersed in a water solution. Proteins could be directly immobilized onto these nanoparticles by a simple coupling process without losing their biological activities. These nanoparticles were further used as fluorescent probes in protein microarray assay for the quantitative detection of protein. The results obtained by these nanoparticles, with the detection limit of as low as 3.5 μg/mL, were much better than those involving the use of conven tional FITC probe.

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LIU Haibo, CHEN Chengxiang, TAN Fang, ZHUANG Zhixia, WANG Xiaoru, YANG Huanghao, YAN Qingpi. A study of using luminophore-doped silica nanoparticles as fluorescent probe in protein microarray assay. Front. Chem. China, 2007, 2(2): 199‒203 https://doi.org/10.1007/s11458-007-0040-6