Rapid Screening and Identification of Brefeldin A in Endophytic Fungi Using HPLC-MS/MS

Abstract

Brefeldin A (BFA; C₁₆H₂₄O₄, MW 280), a naturally occurring macrolide, exhibits diverse biological activities, such as antibiotic, antiviral, cytostatic, antimitotic, and antitumor effects. Owing to the wide activities of BFA, there is a need to develop a method for rapid identification of BFA in crude microbial extracts. In this paper, a method has been established and validated for screening and identification of individual as well as total BFA by high-performance liquid chromatography and liquid chromatography/electrospray mass spectrometry in fungal raw materials.

Keywords

HPLC-MS/MS, endophytic fungi, Brefeldin A, antitumor