Frontiers of Agriculture in China >

2010 , Vol. 4 >Issue 2: 137 - 144

DOI: https://doi.org/10.1007/s11703-010-0110-2

Research articles

Sequence variations of PDHA1 gene in Triticeae species allow for identifying wheat-alien introgression lines

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  • School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China;

Published date: 05 Jun 2010

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Abstract

In order to develop a molecular marker for the detection of alien chromatin, an allele-specific primer targeting pyruvate dehydrogenase (PDHA1) gene was used to analyze 12 taxa representing different basic genomes in Triticeae. Amplification products with different sizes were generated among species. The sequence alignments indicated that the PDHA1 genes contained some deletions/insertions of Miniature Inverted-repeat Terminal Elements (MITE) and simple sequence repeats (SSRs), thus suggesting that the Triticeae genomes have been rapidly evolving during speciation. The genome-specific amplicons and chromosomal location of PDHA1 gene on Triticeae genomes can be used to trace the corresponding alien chromatins from Aegilops, Secale and Dasypyrum species in wheat background.

Key words： PDHA1 gene; Triticeae species; marker assisted selection; phylogeny

Cite this article

Juqing JIA, Guangrong LI, Cheng LIU, Jianping ZHOU, Zujun YANG, . Sequence variations of PDHA1 gene in Triticeae species allow for identifying wheat-alien introgression lines[J]. Frontiers of Agriculture in China, 2010 , 4(2) : 137 -144 . DOI: 10.1007/s11703-010-0110-2

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