Frontiers of Agriculture in China >

2007 , Vol. 1 >Issue 3: 357 - 360

DOI: https://doi.org/10.1007/s11703-007-0060-5

Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain

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  • 1.College of Animal Science and Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China; 2.College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Dingzhou 073000, China

Published date: 05 Sep 2007

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Abstract

The nucleocapsid protein gene of transmissible gastroenteritis virus, 1 149 bp in length, was amplified by RT-PCR from isolated strain HB06 and cloned into pMD18-T. Sequence comparison with other transmissible gastroenteritis virus (TGEV) strains selected from the Gene Bank revealed that the homology of N gene complete sequence shares more than 97% in nucleotide. N gene was cloned into BamHI and EcoRI multiple cloning sites of the prokaryotic expression vector pET 20 b, and named pETN. After being induced by isopropyl-β-D-thiogalactopyranoside (IPTG), the recombinant nucleocapsid protein was expressed. The result of SDS-PAGE and Western-blot showed that the recombinant nucleocapsid protein was 47 kDa and had strong positive reactions with TGEV-specific antibody.

Cite this article

FAN Jinghui, ZUO Yuzhu, LI Tanqing, ZHANG Xiaobo, ZHAO Yuelan . Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain[J]. Frontiers of Agriculture in China, 2007 , 1(3) : 357 -360 . DOI: 10.1007/s11703-007-0060-5

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