In this study, multicolor fluorescence in situ hybridization (FISH) analysis on metaphase chromosomes of spinach with biotin-labeled 25S rDNA, DIG-labeled telomere sequences and biotin-labeled and DIG-labeled 5S rDNA was performed. There were six 25S rDNA loci located on the satellites of the third, the fifth and the sixth chromosomes, and four 5S rDNA loci located on the long arms of the third and the fifth chromosomes. The telomere loci were located on the end of the sixth chromosome and also on both the end and centromeric regions of other chromosomes. This study is an important complement to both traditional karyotype analysis and FISH karyotype analysis in spinach.
LAN Tianying, LIU Bo, DONG Fengping, CHEN Ruiyang, LI Xiulan, CHEN Chengbin
. Multicolor FISH analysis of rDNA and telomere
on spinach[J]. Frontiers of Agriculture in China, 2008
, 2(3)
: 314
-316
.
DOI: 10.1007/s11703-008-0059-6
1. Beech R N, Strobeck C (1993). Structureof the intergenic spacer region from the ribosomal RNA gene familyof white spruce (Picea glauca). Plant Mol Biol, 22(5): 887–892. doi:10.1007/BF00027373
2. Bie S, Wang K B, Wang C Y, Song G L, Kong F L, Liu F, Liu S H, Li S H, Zhang X D, Wang Y H (2004). Studies on 45S rDNA-FISHand karyotype of Gossypium herbaceum and Gossypium arboreum. Cotton Science, 16(4): 223–228 (in Chinese)
3. Chen R Y, Song W Q, Li X L (1979). A new method of plant mitosis chromosomespread. Acta Bot Sin, 21(3): 297–298 (in Chinese)
4. Cox A V, Bennett S T, Parokonny A S, Kenton A, Callimassia M A, Bennett M D (1993). Comparison of plant telomere locationsusing a PCR-generated synthetic probe. Ann Bot, 72(3): 239–247. doi:10.1006/anbo.1993.1104
5. Fuchs J, Brandes A, Schubert I (1995). Telomere sequence localization andkaryotype evolution in higher plants. PlantSyst Evol, 196(4): 227–241. doi:10.1007/BF00982962
6. Hizume M, Kurose N, Shibata F, Kondo K (1998). Molecular cytogenetic studies on sex chromosomes and proximal heterochromatincontaining telomere-like sequence in Cycasrevoluta. Chromosome Sci, 2(1): 63–72
7. Hizume M, Shibata F, Matsusaki Y, Garajova Z (2002). Chromosome identification and comparative karyotypicanalyses of four Pinus species. Theor Appl Genet, 105(4): 491–497. doi:10.1007/s00122‐002‐0975‐4
8. Lengerova M, Kejnovsky E, Hobza R, Macas J, Grant S R, Vyskot B (2004). Multicolor FISH mapping of the dioecious model plant, Silene latifolia. Theor Appl Genet, 108(7): 1193–1199. doi:10.1007/s00122‐003‐1568‐6
9. Liu B, Chen C B, Li X L, Qi L W, Han S Y (2005). Karyotype analysisand physical mapping of 45S rDNA in eight species of Sphora, Robinia and Amorpha. Acta Botanica Yunnanica, 27(3): 261–268 (in Chinese)
10. Meyne J, Baker R J, Hobart H H, Hsu T C, Ryder T C, Ward O G, Wiley J E, Wurster-Hill D H, Yates T L, Moyzis R K (1990). Distribution of non-telocentricsites of the (TTAGGG)n telomeric sequence invertebrate chromosomes. Chromosoma, 99(1): 3–10. doi:10.1007/BF01737283
11. Pedersen C, Linde-Laursen I (1994). Chromosomallocations of four minor rDNA loci and a marker microsatellite sequencein barley. Chromosome Res, 2(1): 65–71. doi:10.1007/BF01539456
12. Qi Z X, Zeng H, Li X L, Song W Q, Chen R Y (2002). The molecular characterizationof maize B chromosome specific AFLPs. CellRes, 12(1): 63–68. doi:10.1038/sj.cr.7290111
13. Regad F, Lebas M, Lescure B (1994). Interstitial telomeric repeats withinthe Arabidopsis thaliana genome. J Mol Biol, 239(2): 163–169. doi:10.1006/jmbi.1994.1360
14. Richards E J, Goodman H M, Ausubel F M (1991). The centromere region of Arabidopsis thaliana chromosome 1 containstelomere-similar sequences. Nucleic AcidsRes, 19(12): 3351–3357. doi:10.1093/nar/19.12.3351
15. Rogers S O, Bendich A J (1987). RibosomalRNA genes in plants: variability in copy number and in the intergenicspacer. Plant Mol Biol, 9(4): 509–520. doi:10.1007/BF00015882
16. Schubert I (1992). Telomeric polymorphism in Viciafaba. Biol Zbl, 111: 164–168
17. Schubert I, Rieger R, Fuchs J (1995). Alteration of basic chromosome numberby fusion-fission cycles. Genome, 38(6): 1289–1292
18. Sone T, Fujisawa M, Takenaka M, Nakagawa S, Yamaoka S, Sakaida M, Nishiyama R, Yamato KT, Ohmido N, Fukui K, Fukuzawa H, Ohyama K (1999). Bryophyte 5S rDNA was inserted into 45S rDNA repeatunits ager the divergence from higher land plants. Plant Mol Biol, 41(5): 679–685. doi:10.1023/A:1006398419556
19. Srivastava A K, Schlessinger D (1991). Structureand organization of ribosomal DNA. Biochimie, 73(6): 631–638. doi:10.1016/0300‐9084(91)90042‐Y
20. Xu Y H, Yang F, Cheng Y L, Ma L, Wang J B, Li L J (2007). Comparative analysis of rDNA distribution in metaphasechromosomes of Cucuibitaceae species. Hereditas(Beijing), 29(5): 614–620 (in Chinese). doi: 10.1360/yc‐007‐0614