RESEARCH ARTICLE

The identification of MacSe in Streptococcus equi ssp. equi

  • Jiande YANG ,
  • Yanfei LIU ,
  • Jun XU ,
  • Jifei MA
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  • Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, China

Received date: 19 Jan 2009

Accepted date: 12 May 2009

Published date: 05 Sep 2009

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

Streptococcus equi subsp. equi (S. equi ssp. equi) causes equine strangles, a highly contagious and widespread purulent lymphadenitis of the head and neck. We have identified MacSe, a novel protein of S. equi, by screening a phage library of 3-8 kb random DNA fragments of S. equi CF32. MacSe shares 62% and 67.5% amino acid homology with Mac5005 and Mac8345 of S. pyogenes respectively. Expression during infection was shown by strong reactivity of the protein with convalescent sera and mucosal wash IgA of ponies infected by commingling exposure. Release into the culture medium was detected during the log phase of growth. Dose dependent anti-phagocytic activity for equine neutrophils involved interaction of MacSe with C3 and neutrophils.

Cite this article

Jiande YANG , Yanfei LIU , Jun XU , Jifei MA . The identification of MacSe in Streptococcus equi ssp. equi[J]. Frontiers of Agriculture in China, 2009 , 3(3) : 304 -310 . DOI: 10.1007/s11703-009-0064-4

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