Somatic embryogenesis and plant regeneration in glandless upland cotton (Gossypium hirsutum L.)

Abstract

Glandless upland cotton has an important economic value. Embryogenic calli and regenerated plants were obtained from the hypocotyl explants of glandless upland cotton seedlings, cultivar Jisheng1. The results indicated that somatic embryogenesis was significantly influenced by the types of auxin and cytokinin. 2, 4-D was advantageous to induce cotton callus, but embryogenic callus could not be obtained on the 2, 4-D medium. Embryogenic calli were also not obtained on the MSB sold medium with the combination of IBA and BA. However, embryogenic calli were induced when the hypocotyl explants were cultured on the IBA and KT medium. More than 31% of the hypocotyl segments produced embryogenic calli when the MSB medium was supplemented with 1.0 mg·L^-1 IBA and 0.5 mg·L^-1 KT. Embryogenic calli with somatic embryos could be observed within three months. Somatic embryo germination and maturation occurred on the hormone-free MSB medium with 1.0 g·L^-1 Gln and 0.5 g·L^-1 Asn. A number of regenerated plants could be obtained in six months. In the present study, a simple and efficient system was established to induce a number of embryogenic calli and regenerate plantlets from hypocotyl explants.

Key words： cotton (Gossypium hirsutum L.); glandless; somatic embryogenesis; plant regeneration

Acknowledgements

This research was supported by the Natural Science Foundation of Hebei Province, China (No. C2007001054).

References

1 Cai X N, Wu J Y, She J M (1997). Embryogenic callus induction and plant regeneration in upland cotton. Jiangsu Journal of Agricultural Sciences, 13(4): 225-230 (in Chinese)

2 Chen Z X, Li S J, Trolinder N L, Goodin J R (1987). Some characteristics of somatic embryogenesis and plant regeneration in cotton cell suspension culture. Sci Agric Sin, 20(5): 6-11 (in Chinese)

3 Davidonis G H, Hamilton R H (1983). Plant regeneration from callus tissue of Gossypium hirsutum L. Plant Sci Lett, 32: 89-93

4 Dong H Z, Jiao G L, Chen Z X (1993). The effect of 2, 4-D and KT on callus initiation and somatic embryogenesis in cotton culture. Acta Agriculturac Boreali-Sinica, 8(3): 87-91 (in Chinese)

5 Guo S G, Li S J, Hu Y X (1996). Tissue culture and plant regeneration of elite cotton species. Journal of Henan University, 26(4): 77-80 (in Chinese)

6 Li G D, Xiao J L, Luo X L (2006). Somatic embryogenesis and plant regeneration of calli derived from cotton ( Gossypium hirsutum L.) depending on their quality. Journal of Shanxi Agricultural Sciences, 34(1): 29-31 (in Chinese)

7 Li J L, Zhang H S, Gao P, Wang H B (1996). Embryogenesis and plant regeneration from somatic cells culture of glandless upland cotton. Cotton Science, 8(4): 223 (in Chinese)

8 Li W M, Zhang H S, Zhao J L, Sun J Y (2008). Breeding and simple-rapid regeneration protocol for Jisheng1 with glandless trait and high-frequency somatic embryo production ability. Cotton Science, 20(Suppl): 95

9 Price H J, Smith R H (1979). Somatic embryogenesis in suspension cultures of Gossypium klotzschianum Anders. Planta, 145: 305-307

10 Sharma A, Kumar A (1994). Somatic embryogenic and plant regeneration from leaf-derived cell suspension of a mature tree: Thevetia peruviana L. Plant Cell Rep, 14: 171-174

11 Wang L Q, Liu F, Zhou Y (2002). Direct somatic embryogenesis and plant regeneration from cotton tissue culture. Cotton Science, 14(6): 340-343 (in Chinese)

12 Wang L Q, Wang M, Shi H R (2004). Hormones regulation on cotton somatic embryogenesis. Letters in Biotechnology, 65(4): 577-579 (in Chinese)

13 Wu J Y, Chen W P, Yin S Z (1988). Effect of exogenous and successive selection on the ability of embryogenesis from long-term culture of G. davidsonii Kell. Jiangsu Journal of Aricultural Sciences, 4(2): 9-15 (in Chinese)

14 Yu Y, Liu C L, Ma Z Y (2004). Embryogenic callus induction and plant regeneration from “CCRI 24”. Acta Botanica Boreali-Occidentalia Science, 24(2): 306-310 (in Chinese)

15 Zhang B H, Guo T L (1996). Studies on cytology and histology of cotton tissue culture. Journal of Hubei Agricultural College, 16(1): 58-66 (in Chinese)

16 Zhang X L, Sun J Z, Liu J L (1991). Somatic embryogenesis and plant regeneration in upland cotton. Journal of Genetics and Genomics, 18(5): 461-467 (in Chinese)

17 Zhang X L, Zhang J M, Yao M J (1993). Studies on somatic cell culture and its application in cotton. Journal of Huazhong Agricultural University, 12(5): 421-426 (in Chinese)