RESEARCH ARTICLE

Biological characteristics of Bacillus thuringiensis strain Bt11 and identification of its cry-type genes

  • Tinghui LIU 1 ,
  • Wei GUO , 1,2 ,
  • Weiming SUN 2 ,
  • Yongxiang SUN 2
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  • 1. College of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071001, China
  • 2. College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China

Received date: 16 Sep 2008

Accepted date: 31 Dec 2008

Published date: 05 Jun 2009

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

A novel strain of Bacillus thuringiensis Bt11, isolated from soil samples in China, was classified and characterized in terms of its crystal proteins, cry genes content. The Bt11 strain showed high toxicity against Spodoptera exigua and Helicoverpa armigera neonates. Bt11 strain shares morphological and biochemical characteristics with the previously described Bacillus thuringiensis subsp. kurstaki. SDS-polyacrylamide gel electrophoresis revealed that crystals were composed of several polypeptides ranging from 20 to 130 kDa, of which the 35, 80, and 130 kDa proteins were the major components. PCR-RFLP with total DNA from strain Bt11 and specific primers for cry1, cry2, cry3, cry4/10, cry7, cry8, cry9, and cry11 genes revealed that cry1Aa, cry1Ab, cry1Ia, and cry9Ea genes were present.

Cite this article

Tinghui LIU , Wei GUO , Weiming SUN , Yongxiang SUN . Biological characteristics of Bacillus thuringiensis strain Bt11 and identification of its cry-type genes[J]. Frontiers of Agriculture in China, 2009 , 3(2) : 159 -163 . DOI: 10.1007/s11703-009-0040-z

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