GC-MS analysis of the chemical constituents of the essential oil from the leaves of yacon (Smallanthus sonchifolia)

Abstract

The essential oil from the leaves of yacon grown in China was isolated by hydrodistillation and distillation-extraction. Chemical constituents of the essential oil were separated and identified by means of gas chromatography/mass spectrometry (GC/MS) for the first time, and the relative content of each constituent was determined by area normalization. Twenty-one chemical constituents were identified, and their amounts accounted for 96.2% of the total composition. The main components of the essential oil were β-phellandrene (26.3%), β-cubebene (17.7%), β-caryophyllene (14.0%) and β-bourbonene (10.2%). Therefore, in the volatile oil from the leaves of yacon, sesquiterpenes are major compounds, accounting for 52.2%.

Key words： GC-MS; chemical constituent; essential oil; Smallanthus sonchifolia

References

1 Adam M, Juklova M, Bajer T, Eisner A, Ventura K (2005). Comparison of three different solid-phase microextraction fibres for analysis of essential oils in yacon (Smallanthus sonchifolius) leaves. Journal of Chromatography A, 1084: 2-6

2 Arctander S (1969). Perfume and Flavor Chemicals (Aroma Chemicals) Vol. 2. New Jersey: Steffen Arctander, 65-67

3 Ceccarini L, Macchia M, Flamini G, Cioni P L, Caponi C, Morelli I (2004). Essential oil composition of Helianthus annuus L. leaves and heads of two cultivated hybrids “Carlos” and “Florom 350”. Ind Crops Prod, 19(1): 13-17

4 Goto K, Fukai K, Hikida J, Nanjo F, Hara Y (1995). Isolation and structural analysis of oligosaccharides from yacon (Polymnia sonchifolia). Biosci Biotechnol Biochem, 59: 2346-2347

5 Guido F, Pier L C, Ivano M, Ammar B (2007). Essential oils of the aerial parts of three Salvia species from Jordan: Salvia lanigera, S. spinosa and S. syriaca. Food Chem, 100: 732-735

6 Inoue A, Tamogami S, Kato H, Nakazato Y, Akiyama M, Kodama O, Akatsuka T, Hashidoko Y (1995). Antifungal melampolides from leaf extracts of Smallanthus sonchifolius. Phytochem, 39(4): 845-848

7 Lachman J, Havrland B, Fernandez E C, Dudjak J (2004). Saccharides of yacon [Smallanthus sonchifolius (Poepp. et Endl.) H. Robinson] tubers and rhizomes and factors affecting their content. Plant Soil EnViron, 50: 383-390

8 Lawless J (1995). The illustrated encyclopedia of essential oils. Dorset: Element Books Ltd, 78-79

9 Newall C A, Anderson L A, Phillipson J D (1996). Herbal Medicines, A Guide for Health-care Professionals. London: The Pharmaceutical Press, 46-48

10 Ohyama T, Ito O, Yasuyoshi S, Ikarashi T, Minamisawa K, Kubota M, Tsukihashi T, Asami T (1990). Composition of storage carbohydrate in tubers of yacon (Polymnia sonchifolia). Soil Sci Plant Nutr, 36(1): 167-171

11 Pedreschi R, Campos D, Noratto G, Chirinos R, Cisneros-Zevallos L (2003). Andean yacon root (Smallanthus sonchifolius Poepp. Endl.) fructooligosaccharides as a potential novel source of prebiotics. J Agric Food Chem, 51: 5278-5284

12 Singh G, Singh O P, Maurya S (2002). Chemical and biocidal. investigations on essential oils of some Indian Curcuma species. Program Cryst Growth Characterization, 45: 75-81

13 Suo M R, Yang J S (2006). Survey in studies on chemical constituents of sesquiterpene and their physiological activities in plants of Helianthus L. Chinese Traditional and Herbal Drug, 37: 135-140 (in Chinese)

14 Valentová K, Frček J, Ulrichova J (2001). Yacon (Smallan-thus sonchifolius) and Maca (Lepidium meyenii), traditional Andean crops as new functional foods on the European market. Chem Listy, 95: 594-601

15 Zardini E (1991). Ethnobotanical notes on ‘yacon’, Polymnia sonchifolia (Asteraceae). Econ Bot, 45: 72-85