Inheritance and molecular markers for the seed coat color in Brassica juncea

Abstract

To elucidate the inheritance of seed coat color in Brassica juncea, Sichuan Yellow inbred (PY) was crossed with the Ziyejie inbred, and their F₁, F₂ and BC₁ and BC₂ progenies, derived from backcrossing to PY, were phenotyped for seed coat color. Results showed that the yellow seed coat was controlled by two independent recessive loci. Seven brown-seeded near-isogenic lines were developed by successive backcrosses to PY and by selfing. One of the BC₆F₂ populations segregated for a single locus controlling seed coat color was used for mapping. Using the 88 primer pairs from sequence-related amplified polymorphism and the 500 random primers, two markers were found to be linked to the gene for brown seed coat, which were designated as SCM57 and SCM1078. The crossover between these markers and the brown seed coat loci was 2.35% and 7.06%, respectively. A sequence characterized amplified region (SCAR) marker according to Negi et al. (2000), designated as SZ1-331, was found to be linked to the gene for brown seed coat, with a cross-over estimate of 2.35%. The markers were located on the same side of the brown seed coat loci and 2.41, 7.51 and 2.41 cM away from the brown seed coat locus. The seven brown-seeded near-isogenic lines were classified into two groups by three DNA markers. They were located at the same linkage group of the marker RA2-A11 previously published by Padmaja et al. (2005).

Key words： inheritance; molecular markers; seed coat color; Brassica juncea

Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (Grant No. 30471098). The experiments were carried out in the pre-state key laboratory for germplasm innovation and resource utilization of crops in China.

References

1 Liu H L (2000). Oilseed Genetics and Breeding. Beijing: China Agricultural University Press, 139-149 (in Chinese)

2 Liu L W, Gong Y Q, Huang H, Zhu X W (2004). Novel molecular marker systems-SRAP and TRAP and their application. Hereditas, 26: 777-781 (in Chinese)

3 Mahmood T, Rahman M H, Stringam G R, Raney J P, Good A G (2005). Molecular markers for seed color in Brassica juncea. Genome, 48: 755-760

4 Michelmore R, Paran W I, Kesseli RV (1991). Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 88(21): 9828-9832

5 Negi M S, Devic M, Delseny M, Lakshmikumaran M (2000). Identification of AFLP fragments linked to seed coat colour in Brassica junceaand conversion to a SCAR marker for rapid selection. Theor Appl Genet, 101: 146-152

6 Padmaja K L, Arumugam N, Gupta V, Mukhopadhyay A, Sodhi Y S, Pental D, Pradhan A K (2005). Mapping and tagging of seed coat colour and the identification of microsatellite markers for marker-assisted manipulation of the trait in Brassica juncea. Theor App1 Genet, 111: 8-14

7 Pradhan A K, Gupta V, Mukhopadhyay A, Arumugam N, Sodhi Y S, Pental D (2003). A high-density linkage map in Brassica juncea(Indian mustard) using AFLP and RFLP markers. Theor Appl Genet, 106: 607-614

8 Sabharwal V, Negi M S, Banga S S, Lakshmikumaran M (2004). Mapping of AFLP markers linked to seed coat colour loci in Brassica juncea(L.) Czern. Theor App1 Genet, 109: 160-166.

9 Vera C L, Woods D L (1982). Isolation of independent gene pairs at two loci for seed coat colour in Brassica juncea. Can J Plant Sci, 62: 47-50

10 Vera C L, Woods D L, Downey R K (1979). Inheritance of seed coat colour in Brassica juncea. Can J Plant Sci, 59: 635-637

11 Yan M L (2004). Molecular biological studies of seed coat color in mustard (Brassica juncea). Dissertation for the Master’s Degree, Changsha: Hunan Agricultural University, 9-11 (in Chinese)