RESEARCH ARTICLE

Cloning and prokaryotic expression of translationally controlled tumor protein (TaTCTP) gene from wheat and preparation of antiserum

  • Lifeng ZHANG ,
  • Aihua YAN ,
  • Dong TIAN ,
  • Shengfang HAN ,
  • Dongmei WANG
Expand
  • College of Life Science, Agricultural University of Hebei, Baoding 071001, China

Received date: 27 Jan 2010

Accepted date: 14 Sep 2011

Published date: 05 Dec 2011

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

The translationally controlled tumor protein (TCTP) is a multi-functioning protein that performs vital roles, particularly in various complicated life processes. To further understand the biological function of translationally controlled tumor protein (TaTCTP) gene from wheat, total RNA was isolated from wheat leaves and then TaTCTP gene was amplified by PCR after reverse transcription by using the anchored primers oligo(dT)18. And then TaTCTP gene was connected into PMD19-T vector for sequence. The recombinant expression vector (pET30a-GST-TaTCTP-His) was constructed and transformed into E. coli strain Rosetta (DE3) subsequently, then a proximate 20 kDa protein in Rosetta (DE3) was expressed and characterized by SDS-PAGE. Moreover, TaTCTP fusion protein was purified by Ni-NTA affinity chromatography from recombinant bacterial lysate and was used to immunize rabbit to produce polyclonal antibody. The titer and specificity of the anti-TaTCTP antibody were successfully detected by indirect ELISA and Western blot analysis.

Cite this article

Lifeng ZHANG , Aihua YAN , Dong TIAN , Shengfang HAN , Dongmei WANG . Cloning and prokaryotic expression of translationally controlled tumor protein (TaTCTP) gene from wheat and preparation of antiserum[J]. Frontiers of Agriculture in China, 2011 , 5(4) : 473 -478 . DOI: 10.1007/s11703-011-1144-9

Acknowledgements

This research was supported by the National Natural Science Foundation of China (Nos. 30671244, 31171472), the Key Basic Research Project of Applied Basic Research Program of Hebei Province, China (No. 08965505D), and the Natural Science Foundation of Hebei Province, China (Nos. C2007000515, C2010000787).
1
Bheekha-Escura R, MacGlashan D W, Langdon J M, MacDonald S M (2000). Human recombinant histamine-releasing factor activates human eosinophils and the eosinophilic cell line, AML14-3D10. Blood, 96(6): 2191-2198

PMID

2
Böhm H, Benndorf R, Gaestel M, Gross B, Nürnberg P, Kraft R, Otto A, Bielka H (1989). The growth-related protein P23 of the Ehrlich ascites tumor: translational control, cloning and primary structure. Biochem Int, 19(2): 277-286

PMID

3
Bonnet C, Perret E, Dumont X, Picard A, Caput D, Lenaers G (2000). Identification and transcription control of fission yeast genes repressed by an ammonium starvation growth arrest. Yeast, 16(1): 23-33

DOI PMID

4
Cans C, Passer B J, Shalak V, Nancy-Portebois V, Crible V, Amzallag N, Allanic D, Tufino R, Argentini M, Moras D, Fiucci G, Goud B, Mirande M, Amson R, Telerman A (2003). Translationally controlled tumor protein acts as a guanine nucleotide dissociation inhibitor on the translation elongation factor eEF1A. PNAS, 100(24): 13892-13897

DOI PMID

5
Cao B H, Lei J J, Chen G J, Zeng G P, Meng C M (2006). Isolation and expression analysis of the gene encoding translationally controlled tumor protein (TCTP) in cabbage. J Agric Biotechnol, 14(6): 996-997 (in Chinese)

6
Chitpatima S T, Makrides S, Bandyopadhyay R, Brawerman G (1988). Nucleotide sequence of a major messenger RNA for a 21 kilodalton polypeptide that is under translational control in mouse tumor cells. Nucleic Acids Res, 16(5): 2350

DOI PMID

7
Dong X, Yang B, Li Y, Zhong C, Ding J (2009). Molecular basis of the acceleration of the GDP-GTP exchange of human ras homolog enriched in brain by human translationally controlled tumor protein. J Biol Chem, 284(35): 23754-23764

DOI PMID

8
Gachet Y, Tournier S, Lee M, Lazaris-Karatzas A, Poulton T, Bommer U A (1999). The growth-related, translationally controlled protein P23 has properties of a tubulin binding protein and associates transiently with microtubules during the cell cycle. J Cell Sci, 112(Pt 8): 1257-1271

PMID

9
Gnanasekar M, Dakshinamoorthy G, Ramaswamy K (2009). Translationally controlled tumor protein is a novel heat shock protein with chaperone-like activity. Biochem Biophys Res Commun, 386(2): 333-337

DOI PMID

10
Graidist P, Yazawa M, Tonganunt M, Nakatomi A, Lin C C, Chang J Y, Phongdara A, Fujise K (2007). Fortilin binds Ca2+ and blocks Ca2+-dependent apoptosis in vivo. Biochem J, 408(2): 181-191

DOI PMID

11
Gross B, Gaestel M, Böhm H, Bielka H (1989). cDNA sequence coding for a translationally controlled human tumor protein. Nucleic Acids Res, 17(20): 8367

DOI PMID

12
Kim M, Jung Y, Lee K, Kim C (2000). Identification of the calcium binding sites in translationally controlled tumor protein. Arch Pharm Res, 23(6): 633-636

DOI PMID

13
Kim M J, Lyu J, Sohn K B, Kim M, Cho M C, Joo C K, Lee K (2009). Over-expression of translationally controlled tumor protein in lens epithelial cells seems to be associated with cataract development. Transgenic Res, 18(6): 953-960

DOI PMID

14
Liu H, Peng H W, Cheng Y S, Yuan H S, Yang-Yen H F (2005). Stabilization and enhancement of the antiapoptotic activity of mcl-1 by TCTP. Mol Cell Biol, 25(8): 3117-3126

DOI PMID

15
Liu W, Yan A H, Hou C Y, Wang D M (2010). Cloning and prokaryotic expression in TaCaM2–3 of wheat and preparation of antiserum. Front Agric China, 4(3): 317-322

DOI

16
Lopez A P, Franco A R (2006). Cloning and expression of cDNA encoding translationally controlled tumor protein from strawberry fruits. Biol Plant, 50(3): 447-449

DOI

17
MacDonald S M, Bhisutthibhan J, Shapiro T A, Rogerson S J, Taylor T E, Tembo M, Langdon J M, Meshnick S R (2001). Immune mimicry in malaria: Plasmodium falciparum secretes a functional histamine-releasing factor homolog in vivo and in vivo. Proc Natl Acad Sci USA, 98(19): 10829-10832

DOI PMID

18
MacDonald S M, Rafnar T, Langdon J, Lichtenstein L M (1995). Molecular identification of an IgE-dependent histamine-releasing factor. Science, 269(5224): 688-690

DOI PMID

19
Nagano-Ito M, Banba A, Ichikawa S (2009). Functional cloning of genes that suppress oxidative stress-induced cell death: TCTP prevents hydrogen peroxide-induced cell death. FEBS Lett, 583(8): 1363-1367

DOI PMID

20
Pay A, Heberle-Bors E, Hirt H (1992). An alfalfa cDNA encodes a protein with homology to translationally controlled human tumor protein. Plant Mol Biol, 19(3): 501-503

DOI PMID

21
Rao K V, Chen L, Gnanasekar M, Ramaswamy K (2002). Cloning and characterization of a calcium-binding, histamine-releasing protein from Schistosoma mansoni. J Biol Chem, 277(34): 31207-31213

DOI PMID

22
Sage-Ono K, Ono M, Harada H, Kamada H (1998). Dark-induced accumulation of mRNA for a homolog of translationally controlled tumor protein (TCTP) in Pharbitis. Plant Cell Physiol, 39(3): 357-360

PMID

23
Sanchez J C, Schaller D, Ravier F, Golaz O, Jaccoud S, Belet M, Wilkins M R, James R, Deshusses J, Hochstrasser D (1997). Translationally controlled tumor protein: a protein identified in several nontumoral cells including erythrocytes. Electrophoresis, 18(1): 150-155

DOI PMID

24
Susini L, Besse S, Duflaut D, Lespagnol A, Beekman C, Fiucci G, Atkinson A R, Busso D, Poussin P, Marine J C, Martinou J C, Cavarelli J, Moras D, Amson R, Telerman A (2008). TCTP protects from apoptotic cell death by antagonizing bax function. Cell Death Differ, 15(8): 1211-1220

DOI PMID

25
Thaw P, Baxter N J, Hounslow A M, Price C, Waltho J P, Craven C J (2001). Structure of TCTP reveals unexpected relationship with guanine nucleotide-free chaperones. Nat Struct Biol, 8(8): 701-704

DOI PMID

26
Xu A, Bellamy A R, Taylor J A (1999). Expression of translationally controlled tumour protein is regulated by calcium at both the transcriptional and post-transcriptional level. Biochem J, 342(3): 683-689

DOI PMID

27
Yan A H, Zhang L F, Zhang Y W, Wang D M (2009). Early stage SSH library construction of wheat near-isogenic line TcLr19 under the stress of Puccinia recondita f. sp. Tritici. Front Agric China, 3(2): 146-151

DOI

28
Yarm F R (2002). Plk phosphorylation regulates the microtubule-stabilizing protein TCTP. Mol Cell Biol, 22(17): 6209-6221

DOI PMID

Outlines

/