Frontiers of Agriculture in China >
Identification of a novel enhancin-like gene from Bacillus thuringiensis
Received date: 28 Feb 2011
Accepted date: 25 Apr 2011
Published date: 05 Dec 2011
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An enhancin-like gene was cloned from Bacillus thuringiensis (Bt) strain GS8 isolated from soil samples in china. The sequence analysis revealed that an open reading frame (ORF) of 2202 nucleotides encoding a protein containing 733 amino acids with a molecular mass of 84 kDa. The enhancin-like protein showed 100% identity to Bel protein (FJ644935) and 23%–41% identity to viral enhancin proteins; in the 252 to 261 amino-acid sequence of enhancin-like protein, a conserved metal binding motif (HEIAH) similar to that in the reported bacterial enhancin-like proteins was found (HEXXH in viral enhancin protein), which indicated that the enhancin-like protein belongs to metalloprotease. The purified enhancin-like protein was fed together with Cry9Ea to Spodopera exigua and Trichoplusia ni larvae, but no significant increase in toxicity was observed.
Key words: Bacillus thuringiensis; enhancin-like gene; Cry9Ea protein; synergism
Dan ZHAO , Wei GUO , Weiming SUN , Daqing XU , Daqun LIU . Identification of a novel enhancin-like gene from Bacillus thuringiensis[J]. Frontiers of Agriculture in China, 2011 , 5(4) : 423 -429 . DOI: 10.1007/s11703-011-1117-z
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