Frontiers of Agriculture in China >
Identification of a gene responsible for the 60-day delay in flowering time of Arabidopsis
Received date: 08 Nov 2010
Accepted date: 25 Nov 2010
Published date: 05 Sep 2011
Copyright
Identification of genes related to flowering-time in Arabidopsis is very important and meaningful contribution to the flowering process control. One late flowering mutant plant, which exhibits 60-day delay in flowering, was screened from Arabidopsis library of T-DNA insertion. Southern blotting was used to confirm the single copy of exogenetic T-DNA in the genome of the mutant. The flanking sequence of T-DNA insert was obtained by TAIL-PCR and then analyzed by BLAST to confirm that the insertion site locates at the sixth exon of AT2G19520.1 (FVE gene). FVE is considered as a classical flowering time gene in Arabidopsis. It is a component of the autonomous pathway that encodes AtMSI4, which is a putative retinoblastoma-associated protein. The late-flowering mutant is named as fve-4, which is similar to fve-3 of Columbia and allelic with fve-1 and fve-2 of Landsberg erecta. The fve-4 mutant’s delay of flowering was longer than that of fve-3 mutant, whose T-DNA insertion is located at the first exon of FVE gene, suggesting that the sixth exon of FVE gene may play a more important role in the control of floral transition.
Key words： delay of flowering; FVE gene; Arabidopsis; gene identification
Jihong XING , Ye ZHANG , Jing ZHANG , Qiaoyun WENG , Jiao JIA , Jingao DONG . Identification of a gene responsible for the 60-day delay in flowering time of Arabidopsis[J]. Frontiers of Agriculture in China, 2011 , 5(3) : 322 -327 . DOI: 10.1007/s11703-011-1084-4
| 1 |
Ausin I, Alonso-Blanco C, Jarillo J A, Ruiz-García L, Martínez-Zapater J M (2004). Regulation of flowering time by FVE, a retinoblastoma-associated protein. Nat Genet, 36(2): 162–166
|
| 2 |
Boss P K, Bastow R M, Mylne J S, Dean C (2004). Multiple pathways in the decision to flower: enabling, promoting, and resetting. Plant Cell, 16(Supp1 l): S18–S31
|
| 3 |
He Y, Michaels S D, Amasino R M (2003). Regulation of flowering time by histone acetylation in Arabidopsis. Science, 302(5651): 1751–1754
|
| 4 |
Jang S, Marchal V, Panigrahi K C S, Wenkel S, Soppe W, Deng X W, Valverde F, Coupland G (2008). Arabidopsis COP1 shapes the temporal pattern of CO accumulation conferring a photoperiodic flowering response. EMBO J, 27(8): 1277–1288
|
| 5 |
Kardailsky I, Shukla V K, Ahn J H, Dagenais N, Christensen S K, Nguyen J T, Chory J, Harrison M J, Weigel D (1999). Activation tagging of the floral inducer FT. Science, 286(5446): 1962–1965
|
| 6 |
Kim H J, Hyun Y, Park J Y, Park M J, Park M K, Kim M D, Kim H J, Lee M H, Moon J, Lee I, Kim J (2004). A genetic link between cold responses and flowering time through FVE in Arabidopsis thaliana. Nat Genet, 36(2): 167–171
|
| 7 |
Kim S, Choi K, Park C, Hwang H J, Lee I (2006). SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. Plant Cell, 18(11): 2985–2998
|
| 8 |
Kole C, Quijada P, Michaels S D, Amasino R M, Osborn T C (2001). Evidence for homology of flowering-time genes VFR2 from Brassica rapa and FLC from Arabidopsis thaliana. Theor Appl Genet, 102(2–3): 425–430
|
| 9 |
Komeda Y (2004). Genetic regulation of time to flower in Arabidopsis thaliana. Annu Rev Plant Biol, 55(1): 521–535
|
| 10 |
Lee H, Suh S S, Park E, Cho E, Ahn J H, Kim S G, Lee J S, Kwon Y M, Lee I (2000). The AGAMOUS-LIKE 20 MADS domain protein integrates floral inductive pathways in Arabidopsis. Genes Dev, 14(18): 2366–2376
|
| 11 |
Lee I, Aukerman M J, Gore S L, Lohman K N, Michaels S D, Weaver L M, John M C, Feldmann K A, Amasino R M (1994). Isolation of LUMINIDEPENDENS: a gene involved in the control of flowering time in Arabidopsis. Plant Cell, 6(1): 75–83
|
| 12 |
Liu Y G, Mitsukawa N, Oosumi T, Whittier R F (1995). Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J, 8(3): 457–463
|
| 13 |
Nyathi Y, De Marcos Lousa C, van Roermund C W, Wanders R J, Johnson B, Baldwin S A, Theodoulou F L, Baker A (2010). The Arabidopsis peroxisomal ABC transporter, comatose, complements the Saccharomyces cerevisiae pxa1 pxa2 Δ mutant for metabolism of long-chain fatty acids and exhibits fatty acyl-CoA-stimulated ATPase activity. J Biol Chem, 285(39): 29892–29902
|
| 14 |
Onouchi H, Igeño M I, Périlleux C, Graves K, Coupland G (2000). Mutagenesis of plants overexpressing CONSTANS demonstrates novel interactions among Arabidopsis flowering-time genes. Plant Cell, 12(6): 885–900
|
| 15 |
Quesada V, Macknight R, Dean C, Simpson G G (2003). Autoregulation of FCA pre-mRNA processing controls Arabidopsis flowering time. EMBO J, 22(12): 3142–3152
|
| 16 |
Schwartz C, Balasubramanian S, Warthmann N, Michael T P, Lempe J, Sureshkumar S, Kobayashi Y, Maloof J N, Borevitz J O, Chory J, Weigel D (2009). Cis-regulatory changes at FLOWERING LOCUS T mediate natural variation in flowering responses of Arabidopsis thaliana. Genetics, 183(2): 723–732
|
| 17 |
Simpson G G (2004). The autonomous pathway: epigenetic and post-transcriptional gene regulation in the control of Arabidopsis flowering time. Curr Opin Plant Biol, 7(5): 570–574
|
| 18 |
Simpson G G, Dean C (2002). Arabidopsis, the Rosetta stone of flowering time? Science, 296(5566): 285–289
|
| 19 |
Simpson G G, Dijkwel P P, Quesada V, Henderson I, Dean C (2003). FY is an RNA 3΄ end-processing factor that interacts with FCA to control the Arabidopsis floral transition. Cell, 113(6): 777–787
|
| 20 |
Tadege M, Sheldon C C, Helliwell C A, Stoutjesdijk P, Dennis E S, Peacock W J (2001). Control of flowering time by FLC orthologues in Brassica napus. Plant J, 28(5): 545–553
|
| 21 |
Waters B M, Chu H-H, DiDonato R J, Roberts L A, Eisley R B, Lahner B, Salt D E, Walker E L (2006). Mutations in Arabidopsis yellow stripe-like1 and yellow stripe-like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds. Plant Physiol, 141(4): 1446–1458.
|
| 22 |
Weigel D, Alvarez J, Smyth D R, Yanofsky M F, Meyerowitz E M (1992). LEAFY controls floral meristem identity in Arabidopsis. Cell, 69(5): 843–859
|
| 23 |
Yan Z Q, Liang D W, Liu H, Zheng G C (2010). FLC: A key regulator of flowering time in Arabidopsis. Russ J Plant Physiol, 57(2): 166–174
|
| 24 |
Zhang J, Xu J X, Kong Y Z, Ji Z D, Wang X C, An F Y, Li C, Sun J Q, Zhang S Z, Yang X H, Mu J Y, Liu X F, Li J Y, Xue Y B, Zuo J R (2005). Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana. Acta Genetica Sinica, 32(10): 1082–1088 (in Chinese)
|
/
| 〈 |
|
〉 |