The control effect of a multifunctional bacterial agent fit for straw amendment against wheat soil-borne diseases

Abstract

Soil-borne diseases of wheat are getting more and more serious in the wheat/maize rotation growing system in northern China. A multifunctional microorganic strain called B1514 was found to have an inhibitory effect against major pathogens of winter wheat soil-borne diseases, have the ability to decompose maize straw, and have the ability to utilize the straw for multiplication. The strain was processed into bacterial agent HAD-1. Field experiments were conducted from 2008 to 2010 to test the control effect of HAD-1 on the major wheat soil-borne diseases, on decomposing ability to maize straw, and on reproductive capacity. Results showed that HAD-1 had significant control effects on sharp eyespot, take-all, and root rot on wheat. The control efficacy at wheat jointing stage was 59.63% to 72.59%, 57.64% to 59.29%, and 54.48% to 63.25%, respectively. The yield loss decreased by 8.67% to 11.70%. The population numbers of the strain B1514 increased 2.68×10⁷–4.83×10⁷ times during the wheat growing season. HAD-1 significantly accelerated the decomposition rate of maize straw in the soil. The decomposition rate increased by 18.7% to 24.3% during wheat growing season.

Key words： Straw amendment; bacterial agent; winter wheat; soil-borne disease; bio-control

Acknowledgements

This research was financially supported by the National Scientific & Technological Support Project (No. 2011BAD16B08).

References

1 Cao K R, Zhu J L (2006). Effects of wheat straw covering and ploughing-free practice on wheat root disease and the quantity of nematode in soils. Plant Protection, 32: 91–93 (in Chinese)

2 Cheng L, You C P, Xiao A P (2003). Advance in the study on antagonistic bacteria. Acta Agriculturae Universitatis Jiangxiensis (Natural Sciences Edition), 25: 732–737 (in Chinese)

3 Cheng X L (2010). Influence of tillage system on wheat root diseases and rhizosphere fungal diversity. Dissertation for the Master Degree. Baoding: Agricultural University of Hebei, 42–43 (in Chinese)

4 Du H, Wang L, Sun B J, Yuan H X, Xing X P, Li H L (2004). Advances in research and development of biological pesticides for control of plant diseases. Journal of Henan Agricultural Sciences, 9: 39–42 (in Chinese)

5 Fang R Y, Zhao H Q, Fang J (2007). Effects of different conservative farming system on winter wheat diseases incidence in Weibei Highland. Chinese Agricultural Science Bulletin, 23: 194–197 (in Chinese)

6 Fravel D (1988). Role of antibiosis in the biocontrol of plant disease. Annual Review of Phytopathology, 26: 75–91

7 Ge H L, Zhao H L, Guo J H (2004). Research and development of micro-biological agri-chemicals for soil-borne diseases. Journal of Anhui Agricultural Sciences, 32: 153–155 (in Chinese)

8 Ji M S, Gu Z M, Zhang Y (2006). Status and Developing Prospects of Biopesticide Research and Application. Journal of Shenyang Agricultural University, 37: 545–550 (in Chinese)

9 Jia C L, Guo H H, Zhang Y, Meng Q H, Yang Q L, Sui X Y (2010). Effects of different seeding manner on the soil structure and wheat seedling growth under maize stalk full returned to the field. Chinese Agricultural Science Bulletin, 26: 243–248 (in Chinese)

10 Jiang Y H, Yu Z R, Ma Y L (2001). The effect of stubble return on agro-ecological system and crop growth. Chinese Journal of Soil Science, 32: 209–213 (in Chinese)

11 Jin Q M, Song S Y, Li H, Su Q F, Zhang W, Wang L X, Sui J (2007). Investigations on soil pathogens quantitative distribution and diseases access from different cultivated types in the maize fields. Journal of Maize Sciences, 15: 93–96 (in Chinese)

12 Lao X R, Wu Z Y, Gao Y C (2002). Effect of long-term returning straw to soil on soil fertility. Transactions of The Chinese Society of Agricultural Engineering, 18(2): 49–52 (in Chinese)

13 Levy E, Gough F J, Berlin K D, Guiana P W, Smith J T (1992). Inhibition of Septoria tritici and other phytopathogenic fungi and bacteria by Pseudomonas fluorescens and its antibiotics. Plant Pathology, 41: 335–341

14 Liu S P, Zhang H C, Dai Q G, Huo Z Y, Xu K, Ruan H F (2005). Effects of no-tillage plus inter-planting and remaining straw on the field on cropland eco-environment and wheat growth. Chinese Journal of Applied Ecology, 16: 393–396 (in Chinese)

15 Luo K, He K, Kuang C F, Zhou Z C (2002). Studies on the inhibition of antagonistic bacteria to tobacco bacterial wilt Ralstonia solanacearum. Chinese Journal of Biological Control, 18: 185–186 (in Chinese)

16 Shen W F, Zhang B X, Shen L R (2004). Classification for antagonistic bacteria CC41 restrict to barley head blight. Acta Agriculturae Zhejiangensis, 16: 84–87 (in Chinese)

17 Shen Y C, Zhang Y B (2000). Biopesticides. Beijing: Chemical Industry Press (in Chinese)

18 Tian S Z, Ning T Y, Wang Y, Li H J, Zhong W L, Li Z J (2010). Effects of different tillage methods and straw-returning on soil organic carbon content in a winter wheat field. Chinese Journal of Applied Ecology, 21: 373–378 (in Chinese)

19 Wang G H, Raaijmakers J M (2004). Antibiotics production by bacterial agents and its role in biological control. Chinese Journal of Applied Ecology, 15: 1100–1104 (in Chinese)

20 Wang J, Lin Q, Ni Y J, Liu Y G, Wang M J (2009). Effects on yield of winter wheat and soil physical-chemical properties under different conservation tillage. Journal of Qingdao Agricultural University (Natural Science), 26: 276–281 (in Chinese)

21 Wu Q L (1993). Wheat roots and stems disease severity grading and reduction loss rate determination. Plant Protection, 19: 44–45 (in Chinese)

22 Xin W (2005). Crops straw microbial degradation and transformation to use. Dissertation for the Master Degree. Jinan: Shandong University, 45–47 (in Chinese)

23 Zhang C Y (2007). Study on allelopathy of maize straw retention on root disease of wheat. Dissertation for the Master Degree. Baoding: Agricultural University of Hebei, 36–39 (in Chinese)

24 Zhang X S, Cao Y S, Cao K Q (2005). Management of wheat soil-borne diseases under the conservative farming system. Journal of Northwest A&F University (Natural Science Edition), 33: 47–48 (in Chinese)

25 Zhang X Z, Xu J H, Li S P (2005). Isolation screening and preliminary identification of plant pathogenic fungi biocontrol strains of Bacillus spp. Soils, 37: 85–88 (in Chinese)

26 Zhao B, He S J (2002). Microbiology Experiment. Beijing: Science Press (in Chinese)

27 Zheng A P, Li P, Wang S Q, Sun H Q (2003). Screening taxonomy of antagonistic strain B34 against Thanatephorus cucumeris. Acta Phytopathologica Sinica, 33: 81–85 (in Chinese)

28 Zhou W Q, Fan H M (2005). Research advance on antagonistic microorganism in biological control. Liaoning Agricultural Sciences, 25: 32–34 (in Chinese)