Frontiers of Agriculture in China >
Construction and transformation for the antisense expression vector of the polyphenol oxidase gene in Yali pear
Received date: 03 Sep 2010
Accepted date: 19 Sep 2010
Published date: 05 Mar 2011
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To inhibit the browning process in fruits of Yali pear, in this paper, antisense gene techniques were used to reduce the expression of BoldItalic gene. A cDNA fragment of 450 bp, which is located at the 3′ terminal of the polyphenol oxidase (BoldItalic) gene, was amplified from Yali pear using the RT-PCR method, then the antisense expression vector was constructed by inserting the fragment of the Yali pear BoldItalic gene between the CaMV promoter and NOS terminator of the expression vector pBI121 in a reverse orientation. After that, with the agrobacterium-mediated method, the BoldItalic antisense gene was transformed into Yali pear shoots. Northern blot analysis and enzyme activity assay showed that the PPO activities in the transgenic Yali pear shoots were significantly decreased, compared with the non-transformed Yali pear shoots. This lays a good foundation for breeding new varieties of pears with browning resistance in the future.
Guiqin LI , Jing QI , Yuxing ZHANG , Zhihua GAO , Dongqian XU , Huixuan LI , Chenmin HUO . Construction and transformation for the antisense expression vector of the polyphenol oxidase gene in Yali pear[J]. Frontiers of Agriculture in China, 2011 , 5(1) : 40 -44 . DOI: 10.1007/s11703-010-1058-y
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