RESEARCH ARTICLE

Construction and transformation for the antisense expression vector of the polyphenol oxidase gene in Yali pear

  • Guiqin LI 1 ,
  • Jing QI 1 ,
  • Yuxing ZHANG , 2 ,
  • Zhihua GAO 1 ,
  • Dongqian XU 1 ,
  • Huixuan LI 1 ,
  • Chenmin HUO 1
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  • 1. College of Biology Science and Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, China
  • 2. College of Horticulture, Agricultural University of Hebei, Baoding 071001, China

Received date: 03 Sep 2010

Accepted date: 19 Sep 2010

Published date: 05 Mar 2011

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

To inhibit the browning process in fruits of Yali pear, in this paper, antisense gene techniques were used to reduce the expression of BoldItalic gene. A cDNA fragment of 450 bp, which is located at the 3′ terminal of the polyphenol oxidase (BoldItalic) gene, was amplified from Yali pear using the RT-PCR method, then the antisense expression vector was constructed by inserting the fragment of the Yali pear BoldItalic gene between the CaMV promoter and NOS terminator of the expression vector pBI121 in a reverse orientation. After that, with the agrobacterium-mediated method, the BoldItalic antisense gene was transformed into Yali pear shoots. Northern blot analysis and enzyme activity assay showed that the PPO activities in the transgenic Yali pear shoots were significantly decreased, compared with the non-transformed Yali pear shoots. This lays a good foundation for breeding new varieties of pears with browning resistance in the future.

Cite this article

Guiqin LI , Jing QI , Yuxing ZHANG , Zhihua GAO , Dongqian XU , Huixuan LI , Chenmin HUO . Construction and transformation for the antisense expression vector of the polyphenol oxidase gene in Yali pear[J]. Frontiers of Agriculture in China, 2011 , 5(1) : 40 -44 . DOI: 10.1007/s11703-010-1058-y

Acknowledgements

The authors acknowledge the financial support of the Science and Technology Planning Project of Hebei Province, China (No. 07220105D-5).
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