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Fertilization in Brassica campestris ssp. pekinensis and its duration of each stage
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This paper reports the process of fertilization in Brassica campestris ssp. pekinensis and the duration of each stage. The results are as follows: (1) Pollen germinates on stigma 2-3 h after pollination. (2) 4-8 h after pollination, pollen tube grows in the style. (3) 8-14 h after pollination, pollen tube grows in the ovary and gets into the ovule via the micropyle. (4) 16 h after pollination, one sperm nucleus moves to the egg and enters it. (5) The sperm nucleus adheres to the nuclear membrane of the egg 18 h after pollination. (6) 20 h after pollination, it enters the egg nucleus and male chromatin gradually disperses and 24 h after pollination, a male nucleolus appears. A large female nucleolus and a small male nucleolus occur in the nucleus of the fertilized egg, and zygote formed. The dispersing of sperm chromatin in the egg nucleus takes about 4 h. (7) 32-34 h after pollination, the division of zygote begins. The dormancy stage of the zygote lasts for about 8-10 h. (8) The pair polar nuclei lie in the chalazal end of the egg before fertilization, which may fuse into a secondary nucleus or not. (9) 16-18 h after pollination, the sperm nucleus moves to the polar nuclei or the secondary nucleus. 18 h after pollination, the sperm nucleus adheres to the nuclear membrane of the polar nuclei or that of the secondary nucleus. (10) 20 h after pollination, the sperm nucleus enters one of the polar nuclei or the secondary nucleus and a triple fusion takes place. The process of fusion is similar to the karyogamy but faster. The dispersing of the sperm chromatin in the polar nucleus or secondary nucleus takes about 2 h. (11) 22 h after pollination, the primary endosperm nucleus formed. The female and male nucleoli cannot fuse with each other before mitotic division of the primary endosperm nucleus. (12) 24 h after pollination, the division of the primary endosperm nucleus actually takes place.
Key words: Brassica campestris ssp. pekinensis; fertilization
Jie PENG , Jiaheng SHEN . Fertilization in Brassica campestris ssp. pekinensis and its duration of each stage[J]. Frontiers of Agriculture in China, 2007 , 1(1) : 62 -66 . DOI: 10.1007/s11703-007-0011-1
[1] |
Chen J (1984). Morphology of Chinese Cabbage. Beijing: Science Press, 78-101 (in Chinese)
|
[2] |
Hu S Y, Zhu C (1979). The fusion of female and male nucleus during fertilization in higher plant. Acta Botanica Sinica, 21(1): 1-10 (in Chinese)
|
[3] |
Hu S Y, Yang H Y (1964). Biology of Angiosperm Fertilization. Beijing: Science Press, 78-115 (in Chinese)
|
[4] |
Li S X (2002). Flower bud differentiation and leaf spheroid formation in Chinese cabbage. Scientia Agricultur Sinica, (6): 36-40 (in Chinese)
|
[5] |
Ma F S (1988). Embryology of Brassica campestris ssp. Pekinensis. In: Botanical Society of China, ed(s). Scientific papers abstract complication of annual meeting of the 55 th anniversary of Botanical Society of China. Beijing: Science Press, 317-318 (in Chinese)
|
[6] |
Ma F S, Meng Z N, Han X D (1986). Studies on reproductive biology in Chinese cabbage. Shandong Agricultural Science, (6): 1-4 (in Chinese)
|
[7] |
Ma F S, Zheng Y J (1992). Embryo development of Brassica campestris ssp. pekinensis. Acta Botanica Boreali-occidentalia Sinica, 12(2): 104-110 (in Chinese)
|
[8] |
Shen J H (1983). Studies on fertilization in soybean. Acta Botanica Sinica, 25(3): 213-218 (in Chinese)
|
[9] |
Wang F Q, Li M, Qu Y M (2001b). Observation on microstructure of male abortion in Chinese cabbage. Chinese Bulletion of Botany, 18(1): 105-109 (in Chinese)
|
[10] |
Wang F Q, Wang C L, Song Z H (2001a). The cytological observation of pollen and anther development in the cabbage 8823 male-sterile line. Acta Botanica Borealioccidentalia Sinica, 21(3): 570-574 (in Chinese)
|
[11] |
Wu S Y (1979). Flowering and seeding of Chinese cabbage. Shandong Agricultural Science, (5): 13-16 (in Chinese)
|
[12] |
Zhang C H, Liu S X, Zhao X Q, Zhang Y J (1991). A study on pollination and fertilization of self-incompatible line Chinese cabbage. Journal of Hebei Agricultural University, 14(3): 30-32 (in Chinese)
|
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