RESEARCH ARTICLE

Presence of indigenous endophytic bacteria in jujube seedlings germinated from seeds in vitro

  • Xiaojie HOU 1 ,
  • Zhengnan LI 2 ,
  • Dangyue HAN 1 ,
  • Qiuxian HUANG 1 ,
  • Longxian RAN , 1
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  • 1. Section Forest Pathology, Forestry College, Agricultural University of Hebei, Baoding 071001, China
  • 2. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071000, China

Received date: 05 May 2010

Accepted date: 18 May 2010

Published date: 05 Dec 2010

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

The presence of indigenous endophytic bacteria in tissue-cultured seedlings germinated from seeds of Zizyphus jujuba var. Fupingdazao was investigated using cultivation, light microscopy and scanning electronic microscopy (SEM). In addition, bacteria specific 16S rDNA PCR followed by denaturing gradient gel electrophoresis (DGGE) was used. No cultivable bacteria could be detected on plates or in liquid cultures. However, large quantities of endophytic bacteria in jujube seedlings were observed under the light microscope. The bacterial cells were round (measuring 1.5 μm-1.8 μm), short rods (2.2 μm-2.9 μm × 1.1 μm-1.9 μm) and rod shaped (3.7 μm-4.4 μm × 1.6 μm-1.9 μm). Rod-shaped bacterial cells (measuring 3.5 m-4.0 m × 1.5 m-2.0 m) were observed by SEM as well. A 16S rDNA fragment of 1.5 kb could be amplified from the total DNA of seedlings of jujube using the bacterial primer pair 27F/1525R. The V3 fragment of 16S rDNA was separated by DGGE, and the 16S rDNA-PCR-DGGE showed that there were at least six dominant bands within the seedlings of Z. jujuba var. Fupingdazao. It can be concluded that endophytic bacteria that cannot be detected by cultivation are present with high densities in jujube seeds.

Cite this article

Xiaojie HOU , Zhengnan LI , Dangyue HAN , Qiuxian HUANG , Longxian RAN . Presence of indigenous endophytic bacteria in jujube seedlings germinated from seeds in vitro[J]. Frontiers of Agriculture in China, 2010 , 4(4) : 443 -448 . DOI: 10.1007/s11703-010-1038-2

Acknowledgements

This research was financially supported by the Foundation for Key Program of Ministry of Education, China (No. 208017), the Natural Science Foundation of Hebei Province, China (No. C2010000675), and the Scientific Foundation of Agricultural University of Hebei, China
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