RESEARCH ARTICLE

Protein extraction from rice (Oryza sativa L.) root for two-dimensional electrophresis

  • Xiaoliang XIANG 1 ,
  • Shuju NING 2 ,
  • Xia JIANG 3 ,
  • Xiaogui GONG 1 ,
  • Renlei ZHU 1 ,
  • Lanfang ZHU 1 ,
  • Daozhi WEI , 1
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  • 1. School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • 2. School of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • 3. College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350002, China

Received date: 06 Apr 2010

Accepted date: 13 May 2010

Published date: 05 Dec 2010

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg

Abstract

An efficient protein extraction method for two-dimensional gel electrophoresis (2-DE) from plant samples is usually challenging due to the low protein content and high level of interfering compounds. Proteomic analyses of rice (Oryza sativa L.) roots are limited by the lack of an efficient protein extraction method. To establish an effective protocol of protein extraction suitable for 2-DE analysis in rice roots, we evaluated three protein extraction methods (trichloroacetic acid [TCA]/acetone, Mg/NP-40/TCA, and tris-base/acetone). Our results showed that the Mg/NP-40/TCA extraction method had the highest protein yield and is the best resolution of protein separation among the three methods. The TCA/acetone method exhibited clear €protein€ profiles€ and €detected€ more €protein €spots with the highest intensity in the region of high Mr (above 45 kDa) than the other methods. However, this method was unable to detect proteins with low-Mr (less than 24.0 kDa). The Tris-base/acetone method showed the poorest resolution of protein separation. Our results suggest that the Mg/NP-40/TCA method was the most effective among the three methods and may provide enhanced proteomic information for rice and other crop roots.

Cite this article

Xiaoliang XIANG , Shuju NING , Xia JIANG , Xiaogui GONG , Renlei ZHU , Lanfang ZHU , Daozhi WEI . Protein extraction from rice (Oryza sativa L.) root for two-dimensional electrophresis[J]. Frontiers of Agriculture in China, 2010 , 4(4) : 416 -421 . DOI: 10.1007/s11703-010-1031-9

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