Effects of regulators on the respiratory enzyme of pear branches during dormant period
Lei BI, Yuxing ZHANG, Bharat Kumar POUDYAL, Xiaolei WU, Jie LIU
Effects of regulators on the respiratory enzyme of pear branches during dormant period
The effects of regulators on the respiratory enzymes of Qiyuesu’s pear branches during dormant period were studied. The results showed that enzyme activities of SDH, G-6-PDH and 6-PGDH were increased by 0.02 mmol/L SA and 120 mg/L GA3. The enzyme activities of phosphohexoisomerase were reduced by 0.02 mmol/L SA and 120 mg/L GA3. Compared with the control, the PGI activity was reduced by 20.5% and 13.6% using 0.02 mmol/L SA and 120 mg/L GA3. Similarly, the SDH activity was increased by 6.1% and 29.2%, respectively. Likewise, the activity of G-6-PDH and 6-PGDH was increased by 93.9% and 24.8%. Changes of respiratory enzymes were consistent with respiratory pathway of regulators, which indicated that SA and GA3 were helpful to break the dormancy by enhancing activities of SDH, G-6-PDH and 6-PGDH.
pear / dormancy / respiratory enzyme
[1] |
Bewley J D, Black M (1994). Seeds: Physiology of Development and Germination. 2nd ed. New York: Plenum, 462
|
[2] |
Bogatek R, Rychter A (1984). Respiratory activity of apple seeds during dormancy removal and germination. Physiol Veg, 22: 181–191
|
[3] |
Brown A P, Wary J L (1968). Correlated change of some enzyme activities and cofactor and substrate contents of pea cotyledon tissue during germination. Biochem J, 108: 437–444
|
[4] |
Erez A (1971). Improved methods for breaking rest in peach and other deciduous fruit species. Jour Am Soc Hort Sci, 96: 519–522
|
[5] |
Faust M, Erez A, Rowland L J (1997). Bud dormancy in perennial fruit trees; physiological basis for dormancy induction maintenance and release. HortScience, 32: 623–629
|
[6] |
Fiehn O, Kopka J, Dormann P, Altmann T, Trethewey R N, Willmitzer L (2000). Metabolite profiling for plant functional genomics. Nat Biotechnol, 18(11): 1157–1161
CrossRef
Google scholar
|
[7] |
Finch-Savage W E, Leubner-Metzger G (2006). Seed dormancy and the control of germination. New Phytol, 171(3): 501–523
CrossRef
Google scholar
|
[8] |
Fuchigami L H, Wisniewski M (1997). Quantifying bud dormancy: physiological approaches. HortScience, 32: 618–632
|
[9] |
Hiatt A J (1961). Preparation and some properties of soluble succinic dehydroge- nase from higher plants. Plant Physiol, 36(5): 552–557
CrossRef
Google scholar
|
[10] |
Hilhorst H W M (1995). A critical update on seed dormancy. I. Primary dormancy. Seed Sci Res, 5(02): 61–73
CrossRef
Google scholar
|
[11] |
Jamieson D J (1998). Oxidative stress responses of the yeast Saccharomyces cerevisiae. Yeast, 14(16): 1511–1527
CrossRef
Google scholar
|
[12] |
Karssen C M, Zagorski Z, Kepczynski J, Groot S P C (1989). Key role for gibbere11ins in the control of seed germination. Ann Bot (Lond), 63: 71–80
|
[13] |
Kong F X, Hu W, Chao S Y, Sang W L, Wang L S (1999). Physiological responses of lichen Xanthoparmelia Mexicana to oxidative stress of SO2. Environ Exp Bot, 42(3): 201–209
CrossRef
Google scholar
|
[14] |
Kuroda H, Sagisaka S, Asada M, Chiba K (1991). Seasonal variation in the activities of NADH-cytochrome c reductase and cytochrome c oxidase in plastids, mitochon-dria and microsomes in apple trees. J Japan Soc Hort Sci, 60(2): 457–466
CrossRef
Google scholar
|
[15] |
Lang G A (1994). Dormancy-The missing links: Molecular studies and integration of regulatory plant and environmental interactions. HortScience, 29: 1255–1263
|
[16] |
Leslie C A, Romani R J (1986). Salicylic acid: a new inhibitor of ethylene biosynthesis. Plant Cell Rep, 5(5): 144–146
CrossRef
Google scholar
|
[17] |
Li B, Foley M E (1995). Cloning and characterization of differentially expressed genes in imbibed dormant and after ripened. Avena fatua embryos. Plant Mol Biol, 29(4): 823–831
CrossRef
Google scholar
|
[18] |
Li Q, Wu Y Y (2004). Biochemistry. Beijing: China Agriculture Press, 143–165 (in Chinese)
|
[19] |
Li X (2004). Control of respiratory metabolism on sweat cherry and studies on technical of dormancy release. The Master's Dissertation. Tai’an: Shandong Agricultural University (in Chinese)
|
[20] |
Li X L, Yuan Z Y, Gao D S (2001). Factors that influence bud dormancy in deciduous fruit trees. J Shandong Univ Nat Sci, 32(3): 386–392 (in Chinese)
|
[21] |
Neales T F, Incoll I D (1968). The control of leaf photosynthesis rate by the level of assimilate concentration in the leaf: a review of the hypothesis. Bot Rev, 34(2): 107–125
CrossRef
Google scholar
|
[22] |
Nir G, Lavee S (1993). Metabolic changes during cyanimide induced dormancy release in grapevines. Acta Hortic, 329: 271–274
|
[23] |
Wang S Y, Jiao J, Faust M (1991). Changes in metabolic enzyme activities during thidiazuron-induced lateral bud break of apple. HortScience, 26: 171–173
|
/
〈 | 〉 |