mRNA level of PKA-c gene in Setosphaeria turcica with different nutrition sources under metal ion or osmotic stress

Abstract

Intracellular signal transduction pathways including MAPK, Ca²⁺, and cAMP signal transduction pathways play important roles in regulating growth, development, and pathogenesis of phytopathogenic fungi. Protein kinase A (PKA) is a key enzyme in cAMP signaling pathway. The transcription level of the gene encoding catalytic subunit of PKA in Setosphaeria turcica under different culture conditions was analyzed by the semiquantitative RT-PCR method. The expression level of PKA-c gene was the lowest on the medium containing sucrose and starch as the carbon source, and it was distinctly inhibited by Cu²⁺, but it was independent of nitrogen source. After the addition of different concentrations of sorbitol, it showed the positive correlation between the inhibition affection and concentrations. However, the highest expression was observed in response to NaCl (0.9 mol/L). This research enriched the biological information resource of filamentous fungi and laid a foundation for the functional analysis about signal transduction pathway in phytopathogenic fungi.

Keywords

Setosphaeria turcica / PKA / gene expression / semiquantitative RT-PCR

References

[1] Doehlemann G, Berndt P, Hahn M (2006). Trehalose metabolism is important for heat stress tolerance and spore germination of Botrytis cinerea. Microbiology, 152(9): 2625-2634

[2] Dürrenberger F, Wong K, Kronstad J W (1998). Identification of a cAMP-dependent protein kinase catalytic subunit required for virulence and morphogenesis in Ustilago maydis. Proc Natl Acad Sci USA, 95(10): 5684-5689

[3] Fan Y S, Cao Z Y, Gu S Q, Dong J G (2004). Effect of different induction factors on appressorium of Setosphaeria turcica. Scientia Agricultura Sinica, 37(5): 769-772

[4] Kronstad J, De Maria A D, Funnell D, Laidlaw R D, Lee N, de Sá M M, Ramesh M (1998). Signaling via cAMP in fungi: interconnections with mitogen-activated protein kinase pathways. Arch Microbiol, 170(6): 395-404

[5] Lee Y H, Dean R A (1993). cAMP regulates infection structure formation in the plant pathogenic fungus Magnaporthe grisea. Plant Cell, 5(6): 693-700

[6] Lemaire K, Van de Velde S, Van Dijck P, Thevelein J M (2004). Glucose and sucrose act as agonist and mannose as antagonist ligands of the G protein-coupled receptor Gpr1 in the yeast Saccharomyces cerevisiae. Mol Cell, 16(2): 293-299

[7] Li C X, Su J, Gong S C, Song X Z, Li G L, Hu G H (2000). Study on Physiological form of corn leaf blight in Heilongjiang Province. Journal of maize Sciences, 8(2): 89-91

[8] Liebmann B, Müller M, Braun A, Brakhage A A (2004). The cyclic AMP-dependent protein kinase a network regulates development and virulence in Aspergillus fumigatus. Infect Immun, 72(9): 5193-5203

[9] Mehrabi R, Kema G H J (2006). Protein kinase A subunits of the ascomycete pathogen Mycosphaerella graminicola regulate asexual fructification, filamentation, melanization and osmosensing. Mol Plant Pathol, 7(6): 565-577

[10] Mitchell T K, Dean R A, 1995, The cAMP-dependent protein kinase catalytic subunit is required for appressorium formation and pathogenesis by the rice blast pathogen Magnaporthe grisea, Plant Cell, 7 (11): 1869-1878

[11] Rossi S, Moreno S (1994). Regulation of protein kinase A subunits during germination of Mucor rouxii sporangiospores. Eur J Biochem, 222(2): 501-506

[12] Sam V V, Johan M T, (2008). Cyclic AMP-protein kinase A and Snf1 signaling mechanisms underlie the superior potency of sucrose for induction of filamentation in Saccharomyces cerevisiae. Eukaryot Cell, 7(2): 286-293

[13] Yamauchi J, Takayanagi N, Komeda K, Takano Y, Okuno T (2004). cAMP-pKA signaling regulates multiple steps of fungal infection cooperatively with Cmk1 MAP kinase in Colletotrichum lagenarium. Mol Plant Microbe Interact, 17(12): 1355-1365

[14] Zhang L Q, Chen Y Q (2006). Effects of different carbon and nitrogen sources and inorganic salts on the culture characteristices of Inontus Obliquus mycelia. Journal of Agricultural Science Yanbian University, 28(1): 41-45 (in Chinese)

[15] Zhou Y L, Tanaka C, Matsushim S, Tsuda M (2003). Protoplast isolation and transformation of Exserohilum turcicum. Chinese Journal of Biotechnology, 19(3): 364-367 (in Chinese)

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg