Establishment of core collection for Chinese tea germplasm based on cultivated region grouping and phenotypic data

Abstract

Tea is an important economic crop in China. About 2665 accessions of tea germplasm (Camellia spp.) are conserved in the China National Germplasm Tea Repository. However, only a small fraction of the collections have been exhaustively used in tea plant improvement programs. The objective of the present study was to develop a core collection of tea plant to enhance the utilization of genetic resources in improvement programs and simplify their management. For this purpose, based on the cultivated region grouping and phenotypic trait data, a core collection of tea plant was established using logarithm proportion and Ward’s cluster method. Approximately 20% of the accessions were then randomly selected from these distinct groups to form a core collection of 532 accessions. Different statistical methods including comparison of mean using Newman-Keuls test, variance using Levene’s test, and frequency distribution using Chi-square test for the traits validated that the variation present in the initial collection was retained in the core collection. The Shannon-Weaver diversity index for different traits was also similar in the core and initial collection. The phenotypic correlations among different quantitative traits that may be under the control of coadapted gene complexes were also preserved in the core collection. This core collection will play an important role in the utilization of tea germplasms.

Keywords

tea plant (Camellia sinensis) / germplasm / core collection / cultivated region / phenotypic trait

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Acknowledgements

This study was supported by the earmarked fund for Modern Agro-industry Technology Research System (Tea), China (CARS-23), Zhejiang Natural Science Foundation Projects, China (No. Y305124), and the National Science and Technology Foundation Platform Project, China (No. 2005DKA21002-08). The authors thank Dr. Sule S. Omolaja at the Cocoa Research Institute of Nigeria and Dr. Lei-Ying Zheng at the Institute of Botany of the Chinese Academy of Sciences, for their critical reviews and constructive comments.

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