Development of EST-SSR markers in peanut (Arachis hypogaea L.)

Abstract

More molecular markers for potential use in peanut genetic research were developed. A total of 92403 EST sequences of peanut (Arachis) in the NCBI database were downloaded and analyzed. 2594 SSRs distributed in 2267 non-redundant EST sequences were detected, with tri-nucleotide motif (65.54%) as the most abundant motif type followed by di-nucleotide motif (28.10%). Among the 92 repeat types, the top eight motif types were AG/TC (20.1%), AAG/TTC (11.8%), AAT/TTA (10.1%), AGG/TCC (6.6%), AGA/TCT (6.3%), AT/TA (5.9%), ACT/TGA (3.8%), and ATG/TAC (3.7%) with higher frequency. A total of 237 primer pairs were successfully designed based on the 2267 SSR-ESTs using DNA star software.

Keywords

peanut / EST-SSR / development

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Acknowledgements

This work was financially supported by the Natural Science Fund of Hebei Province, China (No. C2009000591), Project for the study of Application Foundation of Hebei Province, China (No. 10960120D) and the special fund for Modern Agro-industry Technology Research System, China.

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg