Generation and identification of four new monosomic addition lines of flowering Chinese cabbage-Chinese kale

Abstract

A selection test of four new flowering Chinese cabbage (Brassica campestris L. var. utilis Tsen et Lee)-Chinese kale (Brassica alboglabra Bailey) monosomic alien addition lines was conducted by karyotype analysis and morphological marker identification. The results mainly showed the following: 61‘2n + 1’ plants screened out from 655 progeny plants of the trigenomic hybrid (AAC) backcrossed with its parent flowering Chinese cabbage (AA), four new monosomic alien addition lines(AA+ C₁, AA+ C₅, AA+ C₈ and AA+ C₉) obtained from the 61 ‘2n + 1’ plants, the transmission rates of the monosomic alien addition lines (AA+ C₁, AA+ C₅ and AA+ C₈) of 19.7%, 23.44%, and 36.51% by female gametes and 7.84%, 8.89%, and 9.43% by male gametes, respectively, and the alien chromosome C-1 and C-5 had partial homology with at least one chromosome of A-genome.

Keywords

flowering Chinese cabbage (Brassica campestris L. var. utilis Tsen et Lee) / Chinese kale (Brassica alboglabra Bailey) / monosnomic alien addition lines / transmission rate

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Acknowledgements

This research was supported by the Natural Science Foundation of Hebei Province, China (No. C2009000543).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg