The candidate QTLs affecting phosphorus absorption efficiency and root weight in maize (Zea mays L.)

Abstract

A maize F₂ population was first used to construct a genetic linkage map of Chromosome 6 covering 117.6 cM with an average interval of 3.68 cM between adjacent markers. Based on composite interval mapping (CIM), the quantitative trait loci (QTL) for phosphorus absorption efficiency (PAE) and root-related traits was detected in four environments, i.e., Kaixian County under deficient phosphorus (KXDP), Kaixian County under normal phosphorus (KXNP), SUDP1, and SUDP2. QTLs affecting root weight (RW) were detected simultaneously at the dupssr15 locus region (bin 6.06) on Chromosome 6 in the four environments, while QTL affecting taproot length and fiber number was only detected in one or two environments. The result suggested that taproot length and fiber number were more easily affected by the environment than PAE and RW. The alleles originating from 082 increased PAE and RW on Chromosome 6. The QTL on bin 6.06 explained 4%–10% and 4%–8% of the total phenotypic variance of PAE and RW, respectively, and the estimates of the genetic effects presented dominance and overdominance. The QTL for RW in the dupssr15 locus is the minor QTLs environment interactive effects, which should be particularly useful in MAS manipulation of breeding maize.

Keywords

maize / QTL analysis / candidate QTLs / phosphorus absorption efficiency / root-related traits / four environments

References

[1] Chen J, Cai Y L, Xu L, Wang J G, Zhang W L, Wang G Q, Xu D L, Chen T Q, Lu X G, Sun H Y, Huang A Y, Liang Y, Dai G L, Qin H N, Huang Z C, Zhu Z J, Yang Z G, Xu J, Kuang S F (2011). Identification of QTLs for biomass production in maize (Zea mays L.) under different phosphorus levels at two sites. Front Agric China, 5(2): 152-161

[2] Chen J, Xu L (2011). Comparative mapping of QTLs for H+ secretion of root in maize (Zea mays L.) and cross phosphorus levels on two growth stages. Front Agric China, 5(3): 284-289

[3] Chen J, Xu L, Cai Y, Xu J (2008). QTL mapping of phosphorus efficiency and relative biologic characteristics in maize(Zea mays L.)at two sites.Plant Soil, 313 (1-2): 251-266

[4] Chen J, Xu L, Cai Y, Xu J (2009). Identification of QTLs for phosphorus utilization efficiency in maize (Zea mays L.) across P levels. Euphytica, 167(2): 245-252

[5] Guingo E, RHébert Y, Charcosset A (1998). Genetic analysis of root traits in maize. Agronomie, 18L: 225-235

[6] Helentjaris T, Wright S, Weber D (1986). Construction of a genetic linkage map in maize using restriction fragment polymorphisms. Maize Genet Coop News Lett, 60: 118-120

[7] Hinsinger P (2001). Bioavailability of soil inorganic P in the rhizosphere as affected by root-induced chemical changes: a review. Plant Soil, 237(2): 173-195

[8] Hinsinger P, Gilkes R J (1996). Mobilization of phosphate from phosphate rock and alumina-sorbed phosphate by the roots of ryegrass and clover as related to rhizosphere pH. Eur J Soil Sci, 47(4): 533-544

[9] Kaeppler S M, Parke J L, Mueller S M, Senior L, Stuber C, Tracy W F (2000). Variation among maize inbred lines and detection of quantitative trait loci for growth at low phosphorus and responsiveness to arbuscular mycorrhizal fungi. Crop Sci, 40(2): 358-363

[10] Kosambi D (1944). The estimation of map distances from recombination values. Ann Eugen, 12: 172-175

[11] Stuber C W, Sisco P (1992). Marker-facilitated transfer of QTL alelles between elite inbred lines and responses in hybrids. Proc. 46th Annual Corn and Sorghum Research. Conference, Am. Seed Trade Assoc.,Washington, DC, USA. 104-113

[12] Tuberosa R, Parentoni S, Kim T S, Sanguineti M C, Phillips R L (1998a). Mapping QTLs for ABA concentration in leaves of a maize cross segregating for anthesis date.Maize Genet Coop News Lett, 72: 72-73

[13] Tuberosa R, Salvi S, Sanguineti M C, Maccaferri M, Giuliani S, Landi (2003). Searching for quantitative trait loci controlling root traits in maize: a critical appraisal.Plant and Soil, 255: 35-54

[14] Tuberosa R, Sanguineti M C, Landi P, Giuliani M M, Salvi S, Conti S(2002). Identification of QTLs for root characteristics in maize grown in hydroponics and analysis of their overlap with QTLs for grain yield in the field at two water regimes.Plant Mol Biol, 47(5-6): 697-712

[15] Tuberosa R, Sanguineti M C, Landi P, Salvi S, Casarini E, Conti S (1998b). RFLP mapping of quantitative trait loci controlling abscisic acid concentration in leaves of drought-stressed maize (Zea mays L.).Theor Appl Genet, 97(5-6): 744-755

[16] Wu J X, Jenkins J N, McCarty J C, Zhong M, Swindle M (2007). AFLP marker associations with agronomic and fiber traits in cotton. Euphytica, 153(1-2): 153-163

[17] Yan X, Liao H, Beebe S E, Blair M W, Lynch J P (2004). QTL mapping of root hair and acid exudation traits and their relationship to phosphorus uptake in common bean.Plant Soil, 265(1-2): 17-19

[18] Zhu J, Kaeppler S M, Lynch J P (2005). Mapping of QTL controlling root hair length in maize (Zea mays L.) under deficient phosphorus.Plant Soil, 270: 299-310

[19] Zhu J, Lynch J P (2004). The contribution of lateral rooting to phosphorus acquisition efficiency in maize (Zea mays L.) seedlings.Funct Plant Biol, 31(10): 949-958

[20] Zuber M S (1996). Evaluation of corn root systems under various environments. Proc Annu Corn Sorghum Ind Res Conf, 23: 67-75

Acknowledgements

This research was funded by both Chongqing Key Scientific And Technological Project “Elite Variety Renovation of Rice and Maize”, China (No. CSTC2007AB1045) and Chinese Key Scientific And Technological Project (No. 2006BAD13B03).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg