The physical interaction between LdPLCs and Arabidopsis G beta in a yeast two-hybrid system

Abstract

Phosphoinositide-specific phospholipase C plays pivotal roles in a host of physiologic processes in both animals and plants. Animal PI-PLC is regulated by heterotrimeric G-protein. Plant PI-PLCs are structurally close to the mammalian PI-PLC-ζ isoform, and it is not testified what regulated this isoform enzyme. In this paper, two isoform genes of LdPLC (Pan, 2005) and three subunits of heterotrimeric G-protein in BoldItalic were amplified and recombinated with plasmids of a yeast two-hybrid system. Using this system, we provided the evidence that LdPLC1 and Gβ subunit could be able to interact with each other. This result indicated that LdPLC1 might be regulated by G-protein.

Keywords

phosphoinositide-specific phospholipase C (PI-PLC) / heterotrimeric G-protein / yeast two-hybrid

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Acknowledgements

We are grateful to the Cell Biology Department of College of Life Science, Hebei Normal University, China, for their help in technical assistance. This research was financially supported by the National Natural Science Foundation of China (Grant No. 30570993) and Hebei Natural Science Foundation Program, Hebei Province, China (No. C2008000292).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg