Effects of traditional Chinese medicine “Yimu Shenghuatang” on cytochrome P450 in cow inflammatory endometrial cells

Abstract

To investigate the metabolism mechanism of traditional Chinese medicine “Yimu Shenghuatang” on dairy cow endometrial cells, the primary cultured endometrial cells in dairy cows were isolated by a collagenase digestion method, and an inflammatory model of the endometrial cells by lipopolysaccharide (LPS) was made. The cells were treated with gradient concentrations of LPS, the cultured supernatants were collected in different periods, and the contents of TNF-α, IL-1β were determined by Enzyme-linked immunosorbent assay (ELISA). The results showed that 100 ng·mL^-1 LPS was the optimal concentration to induce the inflammation of cultured endometrial cells. The inflammatory endometrial cells were treated with traditional Chinese medicine “Yimu Shenghuatang” and its main components during the period of 24–72 h, and the expression of cytochrome P450 in cow endometrial cells was determined by Western blot. Our result lays a foundation for treating cow endometritis by using traditional Chinese medicines.

Keywords

dairy cow / inflammatory endometrial cell / CYP450 / traditional Chinese medicine “Yimu Shenghuatang”

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Acknowledgements

This work was supported by the National Science and Technology Foundation of China (No. 2006BAD04A10-4).

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg