Effect of high-temperature stress on the activity of key enzymes in the AsA-GSH cycle in ‘Yali’ pears

Yingli LI, Jianguang ZHANG

Front. Agric. China ›› 2010, Vol. 4 ›› Issue (4) : 463-467.

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PDF(174 KB)
Front. Agric. China ›› 2010, Vol. 4 ›› Issue (4) : 463-467. DOI: 10.1007/s11703-010-1041-7
RESEARCH ARTICLE
RESEARCH ARTICLE

Effect of high-temperature stress on the activity of key enzymes in the AsA-GSH cycle in ‘Yali’ pears

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Abstract

Through treating fruits at higher temperatures, choosing natural fruits on different exposures on a tree canopy and collecting exclusively both sunburn and normal fruits, the metabolic patterns were studied by comparing the activity of key enzymes in the ascorbate-glutathione (AsA-GSH) cycle including ascorbate peroxidase (APX), monodehydroascorbate reductase (MDAR), and glutathione reductase (GR). The results indicated that the activity of three enzymes increased under around less than 35°C acclimating conditions, with no significant changes in membrane electrolyte leakage. The activity of APX, MDAR, and GR rose during the initial stage when fruits were treated at 45°C but decreased significantly as treating time extended. A time response existed in APX, MDAR, and GR to high temperature. For example, APX activity reached the maximum when fruits were treated for 1 h, however, MDAR and GR showed the peak when fruits were treated for 3 h and 5 h, respectively, implying the possible acting sequence relationship of three enzymes in the AsA-GSH cycle. In view of the whole cycle, APX served as the first enzyme directly scavenging active oxygen species, followed by a series of chain reactions to regenerate ascorbic acid (AsA), and GR served as the last post in the cycle. Because various ecological conditions existed in a tree canopy, there was a significant difference in the enzymatic activity among fruits bearing on either exterior or interior canopy. A significantly higher activity of APX and MDAR was found with exterior fruits, compared with interior ones, which may be regarded as a frequent acclimation to high temperature and excessive sunlight.

Keywords

‘Yali’ pear / high-temperature stress / AsA-GSH cycle / antioxidant enzyme / membrane lipid superoxidation

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Yingli LI, Jianguang ZHANG. Effect of high-temperature stress on the activity of key enzymes in the AsA-GSH cycle in ‘Yali’ pears. Front Agric Chin, 2010, 4(4): 463‒467 https://doi.org/10.1007/s11703-010-1041-7

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Acknowledgements

This research was financially supported by the Natural Science Fund of Hebei Province, China: Study on APX metabolic pattern of pear fruits at high temperature and excessive sunlight stress (No. C2010000770).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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