Identification of disease resistances in wheat-Leymus multicaulis derivatives and characterization of L. multicaulis chromatin using microsatellite DNA markers

Abstract

To identify resistance to Fusarium head blight (FHB), cereal yellow dwarf virus (CYDV), stem rust (Sr), and powdery mildew (Pm) in 24 common wheat (Triticum aestivum)-Leymus multicaulis addition/translocation lines that were developed cytogenetically and to verify the authenticity of these lines using microsatellite (SSR) DNA markers. Resistance to FHB was identified in the wheat-L. multicaulis addition lines, Line 9 and Line 26, which both contained L. multicaulis-specific fragments as shown by SSR markers. The translocation line, Trans 1, and the addition lines, Line 5 and Line 29, have resistance to stem rust (IT 0). Resistance to CYDV was evaluated based on virus titers measured by enzyme linked immunosorbent assay (ELISA). The addition line, Line 23, showed low virus titer (0.15), indicating resistance to CYDV. The segregation distribution of CYDV resistance in 98 F₂ plants of Line 23/CS showed a significant deviation from 3∶1. Inoculation with a set of 14 differential Blumeria graminis f. sp. tritici (Bgt) isolates did not detect powdery mildew resistance in translocation line Trans 1, addition line Line 9 and the amphiploid of wheat-L. multicaulis. However, Line 26 exhibited the resistance response pattern of Kavkaz, which carries Pm8, indicating that Line 26 most likely has the powdery mildew resistance gene Pm8 inherited from its parent lines Feng Kang 7 or Feng Kang 10. Twelve SSR markers, distributed on different homeologous chromosome groups of wheat, which distinguished L. multicaulis addition/translocation chromosomes, were used to verify the presence of L. multicaulis chromatin in the putative wheat-L. multicaulis addition/translocation lines. Of the 24 addition/translocation lines investigated using the 12 polymorphic SSR markers, 18 wheat-L. multicaulis derivatives showed the expected L. multicaulis-specific fragments, indicating that all of these 18 addition/translocation lines would most likely have the introgressed L. multicaulis chromosome(s). Chromosomal rearrangements also were detected in some of the wheat-L. multicaulis introgression lines.

Keywords

Triticum aestivum / Leymus multicaulis / ELISA / SSR / disease resistance

References

[1] Anderson J M, Bucholtz D L, Greene A E, Francki M G, Gray S M, Sharma H, Ohm H W, Perry K L (1998). Characterization of wheatgrass-derived barley yellow dwarf virus resistance in a wheat alien chromosome substitution line. Phytopathology, 88(8): 851–855

[2] Ayala L, Hery M, González-de-León D, van Ginkel M, Mujeeb-Kazi A, Keller B, Khairallah M (2001). A diagnostic molecular markers allowing the study of Th. Intermedium-derived resistance to BYDV in bread wheat segregating populations. Theor Appl Genet, 102(6-7): 942–949

[3] Bai G H, Shannar G (1994). Scab of wheat: prospects for control. Plant Dis, 78(88): 760–766

[4] Brettell R I S, Banks P M, Cauderon Y, Chen X, Cheng Z M, Larkin P J, Waterhouse P M (1988). A single wheatgrass chromosome reduces the concentration of barley yellow dwarf virus in wheat. Ann Appl Biol, 113(3): 599–603

[5] Clark M F, Adams A N (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol, 34(3): 475–483

[6] Conti M D, Arcy C J, Jedlinski H (1990). The “yellow plague” of cereals, barley yellow dwarf virus. In: Burnett P A, ed. World Perspectives on Barley Yellow Dwarf Virus. Mexico: CIMMYT, 1–6

[7] Devos K M, Millan T, Gale M D (1993). Comparative RFLP maps of homoeologous group 2 chromosomes of wheat, rye and barley. Theor Appl Genet, 85: 784–792

[8] Dewey D R (1984). The genome system of classification as a guide to intergeneric hybridization with perennial Triticeae. In: Gustafson J P, ed. Gene Manipulation in Plant Improvement. New York: Plenum Press, 209–279

[9] Dong Y S (1986). Study on hybridization of Triticum aestivum with Leymus multicaulis and Leymus racemosus. First Inter Symp Chromosome Engineerings. P1, Xi’an: 185–187

[10] Dubcovsky J, Luo M C , Zhong G Y, Bransteitter R, Desai A J, Kilian A, Kleinhofs A, Dvorák J (1996). Genetic map of diploid wheat, Triticum monococcum L., and its comparison with maps of Hordeum vulgare L. Genetics, 143(2): 983–999

[11] Flor H H (1955). Host-parasite interaction in flax rust-its genetics and other implications. Phytopathology, 45: 680–685

[12] Friebe B, Jiang J, Raupp W J, McIntosh R A, Gill B S (1996). Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica, 91(1): 59–87

[13] Gill K S, Lubbers E L, Gill B S, Raupp W J, Cox T S (1991). A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome, 34(3): 362–374

[14] Gupta K, Balyan S, Edwards J, Isaac P, Korzun V, Röder M S, Gautier M F, Joudrier P, Schlatter R, Dubcovsky J, De La Pena C, Khairallah M, Penner G, Hayden J, Sharp P, Keller B, Wang C, Hardouin P, Jack P, Leroy P (2002). Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theor Appl Genet, 105(2-3): 413–422

[15] Hammond J, ListerR M , Foster J E (1983). Purification, identity and some properties of an isolate of barley yellow dwarf virus from Indiana. J Gen Virol, 64(3): 667–676

[16] Jia J, Zhou R, Li P, Zhao M, Dong Y (2002). Identifying the alien chromosomes in wheat-Leymus multicaulis derivatives using GISH and RFLP techniques. Euphytica, 127(2): 201–207

[17] Jiang J, Gill B S (1994). Nonisotopic in situ hybridization and plant genome mapping: the first 10 years. Genome, 37(5): 717–725

[18] Kong L, Anderson J M, Ohm H W (2005a). Induction of wheat defense and stress-related genes in response to Fusarium graminearum. Genome, 48(1): 29–40

[19] Kong L, Ohm H W, Cambron S E, Williams C E (2005b). Molecular mapping determines that Hessian fly resistance gene H9 is located on chromosome 1AS of wheat. Plant Breed, 124(6): 525–531

[20] Leath S, Heun M (1990). Identification of powdery mildew resistance genes in cultivars of soft red winter wheat. Plant Dis, 74(10): 747–752

[21] Liu J Q, Kolmer J A (1998). Genetics of stem rust resistance in wheat cvs. Pasqua and AC Taber. Phytopathology, 88(2): 171–176

[22] Mujeeb-Kazi A, Bernard M, Bekele G T, Mirand J L (1983). Incorporation of alien genetic information from Elymus giganteus into Triticum aestivum. In: Sakamoto S, ed. Proc 6th Int Wheat Genet Symp. Kyoto: Maruzen, 223–231

[23] Mullan D J, Platteter A, Teakle N L, Appels R, Colmer T D, Anderson J M, Francki M G (2005). EST-derived SSR markers from defined regions of the wheat genome to identify Lophopyrum elongatum specific loci. Genome, 48(5): 811–822

[24] Ohm H W, Anderson J M, Sharma H C, Ayala L, Thompson N, Uphaus J J (2005). Registration of yellow dwarf viruses resistant wheat germplasm line P961341. Crop Sci, 45: 805–806

[25] Peil A, Korzun V, Schubert V, Schemann E, Weber W E, Röder M S (1998). The application of wheat microsatellites to identify disomic Triticum aestivum-Aegilops markgrafii addition lines. Theor Appl Genet, 96(1): 138–146

[26] Peng J H, Lapitan N L V (2005). Characterization of EST-derived microsatellites in the wheat genome and development of eSSR markers. Funct Integr Genomics, 5(2): 80–96

[27] Pestsova E, Ganal M W, Röder M S (2000). Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome, 43(4): 689–697

[28] Plourde A, Comeau A, Fedak G, St-Pierre C A (1989). Intergeneric hybrids of Triticum aestivum × Leymus multicaulis. Genome, 32(2): 282–287

[29] Plourde A, Comeau A, St-Pierre C A (1992). Barley yellow dwarf virus resistance in Triticum aestivum × Leymus angustus hybrids. Plant Breed, 108(2): 97–103

[30] Qi L L, Wang S L, Chen P D, Liu D J, Friebe B, Gill B S (1997). Molecular cytogenetic analysis of Leymus racemosus chromosomes added to wheat. Theor Appl Genet, 95(7): 1084–1091

[31] Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal M W (1998). A microsatellite map of wheat. Genetics, 149(4): 2007–2023

[32] Röder M S, Plaschke J, König S U, Börner A, Sorrells M E, Tanksley S D, Ganal M W (1995). Abundance, variability and chromosomal location of microsatellites in wheat. Mol Gen Genet, 246(3): 327–333

[33] Saghai Maroof M A, Biyashev R M, Yang G P, Zhang Q, Allard R W (1994). Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proc Natl Acad Sci USA, 91(12): 5466–5470

[34] Sharma D, Knott D R (1966). The transfer of leaf-rust resistance from Agropyron to Triticum by irradiation. Can J Genet Cytol, 8(1): 137–143

[35] Shen X, Kong L, Ohm H (2004). Fusarium head blight resistance in hexaploid wheat (Triticum aestivum)-Lophopyrum genetic lines and tagging of the alien chromatin by PCR markers. Theor Appl Genet, 108(5): 808–813

[36] Srnic G, Murphy J P, Lyerly J H, Leath S, Marshall D S (2005). Inheritance and chromosomal assignment of powdery mildew resistance genes in two winter wheat germplasm lines. Crop Sci, 45(4): 1578–1586

[37] Stakman E C, Stewart D M, Loegering W Q (1962). Identification of physiological races of Puccinia graminis var. tritici. US Dep Agric. ARS E, 617: 1–53

[38] Wang X E, Chen P D, Liu D J, Zhang P, Zhou B, Friebe B, Gill B S (2001). Molecular cytogenetic characterization of Roegneria ciliaris chromosome in common wheat. Theor Appl Genet, 102(5): 651–657

[39] Zhang Z Y, Xu J S, Xu Q J, Larkin P, Xin Z Y (2004). Development of novel PCR markers linked to the BYDV resistance gene Bdv2 useful in wheat for marker-assisted selection. Theor Appl Genet, 109(2): 433–439

Acknowledgements

The authors acknowledge financial support by the National Basic Research Program of China (973) (No. 2009CB118301), the National Natural Science Foundation of China (Grant No. 30871514), the Natural Science Foundation of Shandong, China (Grant No. Y2008D31), and USDA-ARS USWBSI (Grant No. 5902069081), and the Purdue University Agricultural Research Programs and USDA-ARS, CRIS (No. 3602-21220-008-03S), and the USDA-ARS CRIS Project (No. 3602-21220-008-00D). We also are grateful to Drs. David Marshall, USDA/ARS, NC, for screening the lines to specific isolates of Blumeria graminis; Junhua Peng, Colorado State University, for identifying EST-derived microsatellites in the wheat EST database; and George Buechley, Department of Botany and Plant Pathology, Purdue University, for stem rust inoculations.

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