The effects of phlorizin stress on the protective enzyme and metabolic regulation substances in the root of M. micromalus

Abstract

The effects of phlorizin stress on the protective enzyme (PE) and metabolic regulation substances (MRS) in the Malus micromalus root were studied by tissue culture method. Under four concentration gradients of phlorizin stress, the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), the content of malondialdehyde (MDA), proline (Pro) and soluble proteins were tested. The results showed that phlorizin stress could lead to different changing tendencies for different detection indicators. The changes of SOD activity, POD activity and Pro content appeared more evident. The difference between the treatments under phlorizin stress and the control without phlorizin stress was not significant in the initial period. With the extension of processing time the difference became more and more evident and the protective enzyme activities and metabolic regulation substances were influenced greatly under different concentrations of phlorizin stress. When the concentration of phlorizin got to 1g·L^−1, the effect was the most obvious. The activities of protective enzymes were significantly improved, with the MDA production reduced, and Pro and soluble protein (SP) accumulation accelerated.