Comparative study on antibiogram of Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp ( Litopenaeus vannamei )

Abstract

This paper compared antibiotic sensitivity between Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp (Litopenaeus vannamei). Recently, white leg shrimp become target species of shrimp culture among shrimp farmers in Malaysia to replace tiger shrimp (Penaeus monodon) culture. However, the baseline information on antibiogram of pathogenic bacteria especially Vibrio spp., the causative agent of vibriosis in white leg shrimp culture is not well established. Therefore, this study was conducted to reveal the antibiogram of Vibrio spp. isolated from diseased postlarval and marketable-sized white leg shrimp. The information gained from this study is useful for shrimp farmers in selecting appropriate antibiotic during disease outbreak. Antibiogram of present bacterial isolates was determined through disk diffusion method against 21 antibiotics (oxolinic acid 2µg, ampicillin 10µg, erythromycin 15µg, furazolidone 15µg, lincomycin 15µg, oleandomycin 15µg, amoxicillin 25µg, colistin sulphate 25µg, sulphamethoxazole 25µg, chloramphenicol 30µg, doxycycline 30µg, florfenicol 30µg, flumequine 30µg, kanamycin 30µg, nalidixic acid 30µg, novobiocin 30µg, oxytetracycline 30µg, tetracycline 30µg, nitrofurantoin 50µg, fosfomycin 50µg, and spiramycin 100µg). A total of 47 Vibrio spp. isolates (Vibrio parahaemolyticus, 24, and V. alginolyticus, 23) from postlarval white leg shrimp and 49 Vibrio spp. isolates (Vibrio parahaemolyticus, 13, Vibrio

Keywords

antibiogram / Vibrio spp / white leg shrimp (Litopenaeus vannamei) / postlarval / marketable size